METTL16在鸡不同类型肌肉中的表达规律及其对肌肉功能的调控作用

旨在了解m6A甲基转移酶样蛋白16(methyltransferase-like protein 16,METTL16)在鸡不同类型肌肉组织中的表达情况及其在鸡骨骼肌功能中的调控作用。本研究利用qRT-PCR技术检测METTL16基因在120日龄广西麻鸡母鸡不同类型肌肉组织中的表达，同时利用siRNA干扰鸡原代成肌细胞中METTL16基因的表达，分析其对成肌细胞增殖、分化和肌纤维形成的影响。结果显示，METTL16基因在鸡的不同类型肌肉组织(胸大肌、胸小肌、缝匠肌、耻坐骨肌内侧肌、耻坐骨肌外侧肌、髂胫外侧肌、腓肠肌内侧肌和背阔肌)中广泛表达，其中，在白肌纤维为主的胸大肌、胸小肌和髂胫外侧肌中表达量相对更高；细胞增殖检测结果显示，干扰METTL16基因表达后，鸡成肌细胞的增殖活力受到了抑制；在成肌细胞分化后干扰METTL16基因表达，鸡成肌细胞RNA m6A甲基化水平呈下降趋势，细胞分化关键基因MyoD表达显著下降(P<0.05),fast-MyHC基因表达显著上升(P<0.001),slow-MyHC基因表达呈下降趋势。综上，METTL16基因在不同类型肌肉中的表达与其肌纤...

Expression of METTL16 in Different Types of Chicken Muscle and Its Regulatory Role in Chicken Skeletal Muscle Function

The purpose of this study was to explore the expression of m6A methyltransferase-like protein 16 (METTL16) in different types of chicken muscle tissue and its regulatory role in chicken skeletal muscle function. In the present study, the tissue expression profile of METTL16 in different muscles of 120-day-old Guangxi partridge hens and the expression of genes related to proliferation, differentiation and muscle fiber formation after siRNA interference by qRT-PCR were analyzed. The results showed that METTL16 gene was widely expressed in different muscle tissues of chickens. White muscles, including major pectoralis, minor pectoralis and lateral iliotibial muscles, have higher METTL16 gene expression. Cell proliferation assay showed that the proliferation activity of chicken myoblasts was inhibited after METTL16 interference. Interfering with METTL16 gene during myoblast differentiation could reduced the methylation level of m6A and slow-MyHC expression in chicken myoblasts, significantly reduced MyoD expression (P<0.05), and significantly elevated fast-MyHC expression (P<0.001). In conclusion, the expression of METTL16 gene in different muscles is related to the composition of muscle fiber types. METTL16 gene may play a significant role in chicken myoblast proliferation and differentiation, and inhibit the formation of fast-twitch muscle fibers. These results indicated that METTL16 might play a crucial role in chicken muscle function regulation.