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应用CRISPR/Cas9技术与分子标记辅助选择创制广东丝苗米新种质
引用本文:王石光,陆展华,刘维,卢东柏,王晓飞,方志强,巫浩翔,何秀英.应用CRISPR/Cas9技术与分子标记辅助选择创制广东丝苗米新种质[J].中国水稻科学,2023,37(1):29-36.
作者姓名:王石光  陆展华  刘维  卢东柏  王晓飞  方志强  巫浩翔  何秀英
作者单位:广东省农业科学院 水稻研究所/广东省水稻育种新技术重点实验室/广东省水稻工程实验室, 广州 510640
基金项目:广东省现代农业产业技术体系资助项目(2021KJ105);广东省重点领域研发计划资助项目(2020B0202090003);广东省重点领域研发计划资助项目(2021B0707010006);广州市基础与应用基础项目(202201010586);广州市基础与应用基础项目(202102080417);广东省重点实验室运行费资助项目(2020B1212060047)
摘    要:【目的】创制优质香型丝苗米水稻新种质,探索水稻育种改良新途径,助力广东丝苗米品牌建设。【方法】以广东省农业主导品种粤农丝苗(YNSM)与优质稻粤王丝苗(YWSM)为材料,利用CRISPR/Cas9基因编辑技术定点编辑上述品种的香味基因Badh2,创制香稻新品系,随后通过分子标记辅助选择(MAS)技术定向导入GW7/GL7位点,系谱选育优质香型丝苗米水稻新种质。【结果】利用CRISPR/Cas9基因编辑技术成功创制两个香稻新品系yn-kobadh2与yw-kobadh2,其2-AP含量均极显著提高,达到239.39~440.79μg/kg,而有效穗数、每穗实粒数、糙米外观品质、千粒重与产量等主要农艺性状均未受到显著影响;MAS技术结合系谱选育的方法成功选育两个丰产性好、籽粒长宽比超过4.3的优质香型丝苗米水稻新品系NWbadh2GW7-1与NWbadh2GW7-2,达到广东丝苗米品种关于香味与外观粒型的认定标准。【结论】利用CRISPR/Cas9基因编辑与分子辅助选择技术相结合可精准、高效地创制新的优...

关 键 词:广东丝苗米  香味  粒形  CRISPR/Cas9  分子标记辅助选择
收稿时间:2022-07-05
修稿时间:2022-09-05

Generating Guangdong Simiao Rice Germplasms by Applying CRISPR/Cas9 Gene Editing and Marker-assisted Selection Technology
WANG Shiguang,LU Zhanhua,LIU Wei,LU Dongbai,WANG Xiaofei,FANG Zhiqiang,WU Haoxiang,HE Xiuying.Generating Guangdong Simiao Rice Germplasms by Applying CRISPR/Cas9 Gene Editing and Marker-assisted Selection Technology[J].Chinese Journal of Rice Science,2023,37(1):29-36.
Authors:WANG Shiguang  LU Zhanhua  LIU Wei  LU Dongbai  WANG Xiaofei  FANG Zhiqiang  WU Haoxiang  HE Xiuying
Institution:Rice Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of New Technology in Rice Breeding / Guangdong Rice Engineering Laboratory, Guangzhou 510640, China
Abstract:【Objective】 To generate new germplasms of high-quality and fragrant Simiao rice, explore new ways of rice breeding and improvement, and build the Guangdong Simiao Rice brand. 【Method】 Using the leading variety Yuenongsimiao (YNSM) and high-quality rice variety Yuewangsimiao (YWSM) as materials, the fragrance gene Badh2 of these varieties was knocked out by CRISPR/Cas9 gene editing technology to generate new fragrant rice lines. Subsequently, the excellent GW7/GL7 was introduced into these new fragrant rice lines by marker-assisted selection (MAS). 【Result】 Two new fragrant rice lines, yn-kobadh2 and yw-kobadh2, were successfully generated by CRISPR/Cas9 gene editing technology. 2-AP contents of these lines were significantly increased, ranging from 239.39 to 440.79 μg/kg, other main agronomic traits, including effective panicle number, grain number per panicle, appearance quality of brown rice, 1000-grain weight, and grain yield, were not significantly affected. Two novel fragrant Simiao rice lines, NWbadh2GW7-1 and NWbadh2GW7-2, with high grain yield and slender grain size of length to width ratio more than 4.3, were selected by MAS combined with pedigree breeding, which met the identification standards of Guangdong Simiao rice varieties on aroma and appearance grain size. 【Conclusion】 Combination of CRISPR/Cas9 gene editing and MAS technology contribute to accurate and efficient breeding of new excellent rice germplasms. The new germplasms developed can provide important germplasms and genetic resources for breeding of new high-quality fragrant Simiao Rice varieties in Guangdong Province.
Keywords:Guangdong Simiao rice  aroma  grain shape  CRISPR/Cas9  marker-assisted selection  
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