| 褪黑素单克隆抗体的制备 |
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| 引用本文： | 武海新,廖晨星,常毅,马慧,刘巧香,李有志,郭刚,刘国世. 褪黑素单克隆抗体的制备[J]. 中国乳业, 2023, 0(2): 20-25. DOI: 10.12377/1671-4393.23.02.04 |
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| 作者姓名： | 武海新 廖晨星 常毅 马慧 刘巧香 李有志 郭刚 刘国世 |
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| 作者单位： | 1 北京市中国农业大学动物科学技术学院,北京 100193;2 北京首农食品集团有限公司,北京 100028;3 山东省饲料兽药质量检验中心,山东济南 250109 |
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| 摘 要： | 褪黑素（MT）广泛存在于哺乳动物体内,是一种常见的吲哚类物质,可起到清除自由基、调节免疫、调节神经等作用。本文使用褪黑素-OVA作为免疫原,免疫5 只BALB/c小鼠,5 次免疫后采血检测,通过间接ELISA方法确定抗血清针对褪黑素-OVA的滴度。经ELISA筛选,最终获得5 株可稳定表达褪黑素抗体的单克隆细胞株（3G3B2、3G3C7、3G3D1、3G3F4、3G3G3）。纯化后抗体浓度为0.28 mg/mL,经测定,褪黑素单克隆抗体滴度可达1∶64 000,为进一步开发褪黑素的快速检测方法奠定了材料基础。
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| 关 键 词： | 褪黑素 单克隆抗体 免疫 细胞融合 ELISA |
Production of Monoclonal Antibody Against Melatonin |
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| WU Haixin,LIAO Chenxing,CHANG Yi,MA Hui,LIU Qiaoxiang,LI Youzhi,GUO Gang,LIU Guoshi. Production of Monoclonal Antibody Against Melatonin[J]. China Dairy, 2023, 0(2): 20-25. DOI: 10.12377/1671-4393.23.02.04 |
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| Authors: | WU Haixin LIAO Chenxing CHANG Yi MA Hui LIU Qiaoxiang LI Youzhi GUO Gang LIU Guoshi |
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| Affiliation: | 1College of Animal Science and Technology,Beijing China Agricultural University,Beijing 100193;2Beijing Capital Agribusiness& Foods Group,Beijing 100028;3Shandong Feed and Veterinary Drug Quality Inspection Center,Jinan Shandong 250109 |
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| Abstract: | Melatonin （MT）,which is widely existed in mammals,is a common indole substance that can play a role in scavenging free radicals,regulating immunity,and regulating nerves. Here,five BALB/c mice were immunized with melatonin -OVA as the immunogen,and blood was collected for detection after five immunizations. The titer of antiserum against melatonin-OVA was determined by indirect ELISA. After ELISA screening,5 monoclonal cell lines（3G3B2,3G3C7,3G3D1,3G3F4 and 3G3G3） stably expressing melatonin antibody were obtained. The concentration of purified antibody was 0.28 mg/mL,and the titer of purified antibody reached 1∶64 000. The melatonin monoclonal antibody produced in this research laid a material foundation for further development of a rapid detection method of melatonin. |
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| Keywords: | melatonin monoclonal antibody immunity cell fusion ELISA |
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