| CRISPR/Cas9系统在寄生原虫基因编辑中的应用 |
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| 引用本文： | 邓敏儿,李娜,郭亚琼,冯耀宇,肖立华. CRISPR/Cas9系统在寄生原虫基因编辑中的应用[J]. 畜牧兽医学报, 2023, 54(1): 69-79. DOI: 10.11843/j.issn.0366-6964.2023.01.007 |
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| 作者姓名： | 邓敏儿 李娜 郭亚琼 冯耀宇 肖立华 |
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| 作者单位： | 华南农业大学, 岭南现代农业科学与技术广东省实验室, 广州 510642 |
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| 基金项目： | 广东省普通高校创新团队项目(2019KCXTD001)；国家自然科学基金项目(32030109) |
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| 摘 要： | 原虫生活史复杂，大部分难以通过体外培养完成整个生活史，缺乏高效的基因编辑系统。CRISPR/Cas9系统可以通过人工设计的方式，实现基因的精确、高效、快速编辑，被广泛应用于基因工程各个领域。CRISPR/Cas9系统为原虫的未知功能基因研究开辟了新途径。本文介绍CRISPR/Cas9系统的原理和目前在寄生原虫中的基因定位、基因功能研究、高通量基因家族筛选等方面的应用。
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| 关 键 词： | CRISPR/Cas9 基因编辑 寄生虫 原虫 |
| 收稿时间： | 2022-05-18 |
Application of CRISPR/Cas9 System on Gene Editing of Parasitic Protozoa |
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| DENG Min'er,LI Na,GUO Yaqiong,FENG Yaoyu,XIAO Lihua. Application of CRISPR/Cas9 System on Gene Editing of Parasitic Protozoa[J]. Chinese Journal of Animal and Veterinary Sciences, 2023, 54(1): 69-79. DOI: 10.11843/j.issn.0366-6964.2023.01.007 |
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| Authors: | DENG Min'er LI Na GUO Yaqiong FENG Yaoyu XIAO Lihua |
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| Affiliation: | Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | Protozoa have complex life cycle and most of them can not complete the life cycle just in vitro. As a result, most of them lack an effective gene editing system. The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (endonuclease) system facilitates the precise, efficient, and rapid genetic editing through homologous recombination, and is widely used in genetic modification of eukaryotic organisms. The CRISPR/Cas9 system opens up a new avenue for targeted investigation of gene functions in protozoans. This article introduces the principle of the CRISPR/Cas9 system and its major applications in gene tagging and depletion and genome-wide screening of functional gene families in parasitic protozoan. |
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| Keywords: | CRISPR/Cas9 genetic editing parasite protozoa |
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