西番莲水通道蛋白PePIP2基因克隆及表达分析

西番莲作为新兴的热带果树,在热带农业中具有非常重要的作用,然而干旱、高温等非生物胁迫严重影响其正常生长发育。相关研究表明水通道蛋白(AQP)能够提高植物的抗逆性,本研究以西番莲‘台农’(Passiflora edulia Sims)为实验材料,利用西番莲的基因组数据,通过同源克隆法从西番莲中克隆得到了一个水通道蛋白基因PePIP2,其开放阅读框(open reading frame,ORF)为861 bp,编码286个氨基酸。对其进行生物信息学分析,其分子式为C₁₄₁₇H₂₁₅₆N₃₆₀O₃₇₃S₈,预测分子量为30 459.37 Da,等电点为8.84,亚细胞定位于细胞膜上。启动子区域分析显示其含有参与胁迫应答的顺式作用元件。对其进行干旱、高盐、低温、高温胁迫下的表达分析,结果表明：PePIP2能够受到干旱、高温和低温胁迫的诱导表达,其中在土壤含水量为50%,在45℃高温处理4 h和0℃低温处理48 h,其表达量最高。在烟草中进行瞬时表达,在不同时间的干旱胁迫处理下,PePI...

Cloning and Expression Analysis of PePIP2 Gene from Passiflora edulia Sims

Passion fruit (Passiflora edulia Sims), as an emerging tropical fruit tree, plays a very important role in tropical agriculture. However, abiotic stresses such as drought and high temperature seriously affect its normal growth and development. Relevant studies have shown that aquaporin (AQP) can improve the stress resistance of plants. In this study, Passiflora ‘Tainong’ was used as the material, an aquaporin gene PePIP2 was cloned using the genome data of passion fruit. The open reading frame (ORF) was 861 bp, encoding 286 amino acids. The molecular formula was C₁₄₁₇H₂₁₅₆N₃₆₀O₃₇₃S₈, the molecular weight was 30 459.37 Da, the isoelectric point was 8.84, and the subcellular localization was on the cell membrane. Analysis of the promoter region revealed that it contained cis-acting elements involved in stress response. The expression analysis showed that PePIP2 could be induced by drought, high temperature and low temperature stress. Among them, the expression level was the highest when the soil water content was 50%, treated at 45℃ for 4 h and at 0℃ for 48 h. Transiently expressed in tobacco, the expression of PePIP2 was also induced to varying degrees under drought stress at different times. The results of this study would lay the foundation for the analysis of the stress resistance mechanism and molecular breeding of the passion fruit.