西瓜植株再生优化体系的研究

为了将重组基因转入西瓜体内,首先需要建立植株再生体系.以甜王七号﹑超甜京欣王和早佳3个西瓜品种幼苗为材料,研究培养基种类、6-BA与NAA浓度组合、苗龄、外植体类型以及光暗条件对组织再生的影响,提出西瓜植株再生优化体系.结果表明,西瓜组织不定芽诱导率约为 67%～87%,MS培养基能较好地满足西瓜不定芽诱导,1～2 mg·L-1 6-BA与0.1～0.3 mg·L-1 NAA配合有利于不定芽再生.西瓜组织再生以子叶近胚端较好,苗龄以淡黄色转为淡浅绿色为好.研究发现,下胚轴可以直接诱导不定芽产生,其诱导率为 63%,远远高于从愈伤组织再分化的诱导率.不定芽置于1/2MS+NAA 0.1 mg·L-1中生根可形成为完整再生植株.

Optimized system for plant regeneration of watermelon

In order to transfer recombinant genes into watermelon(Citrullus lanatus Mansfeld)to breed new germ plasma,it is necessary to establish plant regeneration system firstly.Based on previous studies,an optimized regeneration system for watermelon was demonstrated with young seedlings of watermelon(cv.Tianwang No 7,Supersweet wang jingxin and Zaojia)to compared effects of medium types,combinations of 6-BA and NAA,seedling ages,explant types and darkness.The results showed that the best induction efficiency was ...