苦玄参育种材料遗传多样性SSR分析

目的]探索苦玄参育种材料的遗传多样性及亲缘关系,为苦玄参育种资源的选择及遗传改良提供参考依据.方法]利用SSR分子标记,分析12份苦玄参育种材料的基因多样性指数(Nei)、Shannon多态性信息指数(Ⅰ)、多态性百分率(P)及各材料间的遗传距离,并基于遗传距离对其进行聚类分析,以明确该育种材料的遗传多样性和亲缘关系.结果]17对SSR引物共扩增出73个等位基因,平均每对引物4.3个.各引物间P变化范围为8.33%~100.00%,平均60.78%;多态信息量(pIC)变化范围为0.0767~0.7598,平均0.5184.供试材料遗传多样性的平均Nei=0.3002、I=0.4162、P=59.81%,其中xyj01、xyj06和ytL09种质Nei、I和P最高,分别为0.3529、0.4893和70.59%;Lxj 12种质Nei、I和P最低,分别为0.2059、0.2854及41.18%.ytl09和zgb03 2份种质间遗传距离最近,仅0.0929.基于遗传距离的聚类分析结果表明,在遗传距离0.4084处可将12份种质分成4个群体,其中wzhjx07、lxj09和xyj01自成一群,其余归为一群.Lxjl2与zgb04、ytll 1与wzhjx09、ytl09与zgb03、wzhjxl2与xyj06和wzhzp22间分别拥有较近的亲缘关系.结论]供试12份苦玄参种质材料间存在一定的遗传差异,作为育种亲本具有一定的选择价值.

SSR analysis on genetic diversity in Picria felterrae Lour.breeding materials

Objective]The objective of this research was to explore genetic diversity and genetic relationship of Picria felterrae Lour.breeding materials and to provide reference for breeding materials selection and genetic improvement.Method]Using SSR molecular marker,gene diversity index (Nei),shannon diversity information index (I),percentage of polymorphic loci (P) and genetic distance of 12 breeding materials P.felterrae Lour.were analyzed.And cluster analysis was conducted based on genetic distance to study the genetic diversity and genetic relationship of the 12 breeding materials.Result]A total of 73 alleles were amplified from 17 pairs of SSR primers,4.3 alleles per primer.Among the primers,P varied between 8.33％-100.00％,with an average of 60.78％;polymorphic information content(PIC) varied between 0.0767-0.7598 which was 0.5184 on average.For the 12 germplasm samples tested,the average level of genetic diversity was Nei=0.3002,I=0.4162,and P=59.81％.Number xyj01,xyj06 and ytL09 had the highest Nei which was 0.3529,the highest I 0.4893 and the highest P 70.59％.Whereas Lxjl2 had the lowest level in Nei which was 0.2059,the lowest I 0.2854 and the lowest P 41.18％.Number ytl09 and zgb03 had the shortest genetic distance,which was 0.0929.The cluster analysis based on genetic distance revealed that the 12 germplasm samples could be divided into four groups at the location where genetic distance was 0.4084.Number wzhjx07,lxj09,and xyj01 made up three groups by themselves,and the rest was in the last group.There were close genetic relationships between Lxj12 and zgb04,between ytl11 and wzhjx09,between ytl09 and zgb03,between wzhjx12 and xyj06,wzhzp22.Conclusion]Certain genetic difference exists in the 12 tested Picria felterrae Lour.breeding materials,which are valuable in terms of selection of parents in breeding.