玉米3种非组培转基因方法转化外源bar基因研究

本试验用3种非组培型转基因方法，即花粉介导、子房注射、萌动种胚法在玉米上转化bar基因，经大田筛选及PCR和PCR-Southern检测，证明均可获得转化植株。还分析了3种方法的转化机理，并通过转化率与操作简便程度的比较，认为花粉介导优于萌动种胚法，二者又优于子房注射法。

Comparison of Three Non-tissue Culture Plant Transformation Approaches

At present, high velocity microprojectile and Agrobacterium tumeficiens infection are two main methods to transform cereal such as wheat and maize. The former is not only expensive, but also with low transgenic and high infertile frequency, and the later also takes immature embryo as the object, spending much time in tissue culture, and is with low transgenic frequency too. Although acetosyringone has been introduced into Agrobacterium tumeficiens infection, the transgenic frequency is still not high. At the same time, all these transgenic experiments can only be done in instrumented laboratory by few scientists. In this study, three non-tissue culture methods were used to transform foreign bar gene into maize. T₁ seeds were identified by Basta resistance, RCR amplification and Southern blotting, T₂ genetic rate and phenotype, in order to select a simple highly effective transgenic approach.