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| 绿色荧光蛋白基因重组子的构建及其在鱼类受精卵中的表达 | |
| 引用本文： | 苏建明. 绿色荧光蛋白基因重组子的构建及其在鱼类受精卵中的表达[J]. 水产学报, 2003, 27(5): 409-414 |
| 作者姓名： | 苏建明 |
| 作者单位： | 1. 湖南农业大学动物科技学院,湖南,长沙,410128 2. 中南林学院,湖南,长沙,412006 3. 北京市水产科学研究所,北京,100075 |
| 基金项目： | 北京市水产科学研究所合作课题，北京市计委资助项目(2001JJN230) |
| 摘 要： | 通过体外重组，将绿色荧光蛋白基因编码序列克隆到鲤鱼肌动蛋白基因启动子下游，构建成能在鱼体内表达绿色荧光蛋白的重组分子。用限制性内切酶PvuI酶切线性化后，通过显微注射法导入金鱼受精卵内，在转化后48h，可观察到绿色荧光，经PCR初步筛选后，对显阳性个体进行Southern杂交检测，结果表明，转化个体表现出较强的杂交信号。这说明绿色荧光蛋白基因能在金鱼体内整合、表达。 |
| 关 键 词： | 绿色荧光蛋白 鲤鱼 基因重组 金鱼 表达 受精卵 |
| 文章编号： | 1000-0615(2003)05-0409-06 |
| 收稿时间： | 2014-03-16 |
| 修稿时间： | 2002-11-25 |
Construction of green fluorescent protein gene recombinant and its expression in fishes zygotes |
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| SU Jian-ming. Construction of green fluorescent protein gene recombinant and its expression in fishes zygotes[J]. Journal of Fisheries of China, 2003, 27(5): 409-414 | |
| Authors: | SU Jian-ming |
| Affiliation: | SU Jian-ming~1,ZHANG Huai-yun~2,XIAO Tiao-yi~1,ZHANG Xue-wen~1,CHEN Tao~1,SU Jian-tong~3,WANG Yue-zhi~3 |
| Abstract: | The coding sequence of green f luorescent protein gene was cloned under the control of carp ..-actin gene promoter. A fish expression of green fluorescent protein gene recombinant was constructed. The recombinant gene was transferred into fertilized zygotes of goldfish ( Carassius auratus) via micro injection after Pvu .. linearing . The green f luorescence was visualized within 48 h following transformation. The transformation and expression of green fluorescent protein was demonstrated by PCR and Southern blotting. In Southern hybridization analysis, transformations got positive signals. The results indicated that the green fluorescent protein gene had integrated into goldfish genome. |
| Keywords: | green fluorescent protein gene recombinant Carassius auratus expression |
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