鸡MxA全基因的克隆及在大肠杆菌中的表达

MxA是由Ⅰ型干扰素诱导宿主细胞所产生的抗病毒蛋白家族的成员之一。本文以Poly:IC和干扰素联合诱导鸡成纤维细胞，使MxA基因活化并转录mRNA，然后从细胞中提取总RNA，并进行RT-PCR，扩增MxA基因，序列分析后克隆到pGEX原核表达载体中，在大肠杆菌中进行诱导表达。表达产物经复性、纯化回收后，得到浓度为10mg/ml较纯的MxA重组蛋白。经体内、体外活性检测实验表明，此表达产物具有阻断新城疫病毒感染的功能,具有很好的研发前景。

Cloning and expression of MxA gene from chickens in E.COLI

MxA was a new kind of antivirus protein that was induced by IFNα/β.It’s very useful and necessary to study for the mankind. We induced CEF with IFNβand Poly:IC in order to get MxA gene. Then we abstracted total RNA from the CEF. A pair of primers was designed to carry through RT-PCR. The PCR product was sequenced and then they were inserted into a expression plasmid vector. The full-length recombination MxA protein was got by induced E.coli .Then we puried and disposed the product in order to get the active recombination protein.We can know the density of the MxA was 10mg/ml by analyse. The active experiment done in and out of the body proved that the recombination MxA protein had biological activity of interferon virus .We have enough reasons to believe that the recombination MxA protein will has a good and useful prosepect.