A preliminary genetic analysis of fibre traits and the use of new genomic SSRs for genetic diversity in jute |
| |
Authors: | Reyazul R Mir Sachin Rustgi Shailendra Sharma Ravinder Singh Aakash Goyal Jitendra Kumar Anupama Gaur Akhilesh K Tyagi Haseena Khan Mohit K Sinha Harindra S Balyan Pushpendra Kumar Gupta |
| |
Institution: | (1) Molecular Biology Laboratory, Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut, 250 004, Uttar Pradesh, India;(2) Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110 021, India;(3) Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh;(4) Division of Crop Improvement, Central Research Institute for Jute and Allied Fibres, Barrackpore, 743 101, West Bengal, India |
| |
Abstract: | Jute is one of the most important fibre crops, which is second only to cotton in providing environment-friendly (biodegradable
and renewable) ligno-cellulose fibre. In order to improve this largely neglected crop, we conducted a preliminary study involving
the following: (i) analysis of nature and extent of the genetic variability for fibre yield and four other related traits
in a set of 81 genotypes belonging to two commercially cultivated Corchorus species (45 genotypes of C. olitorius + 36 genotypes of C. capsularis), (ii) development and analysis of a set of simple sequence repeat (SSR) markers from C. olitorius, and (iii) use of a sub-set of SSRs for assessment of genetic diversity in the above set of 81 genotypes. The results suggested
quantitative nature of fibre yield and other related traits, with a preponderance of dominance component in genetic variance.
A sub-set of 45 SSRs derived from C. olitorius, when used for a study of DNA polymorphism and genetic diversity, showed high transferability of these C. olitorius SSRs to C. capsularis. The average number of alleles for individual SSRs was surprisingly low (3.04 for both species, 2.02 for C. capsularis and 2.51 for C. olitorius), and so was the average polymorphic information content (PIC; 0.23 and 0.24 in two species). In the dendrogram obtained
using a similarity matrix, the 81 genotypes were grouped into three clusters, which largely corresponded to the two species,
Cluster I belonging mainly to C. capsularis and the other two closely related clusters (clusters II and III) belonging to C. olitorius. It was also shown that a minimum of 15 SSRs could give the same information as 41 SSRs, thus making many SSRs redundant.
The SSR markers developed during the present study and to be developed in future will prove useful not only for evaluation
of genetic diversity, but also for molecular mapping/QTL analysis, and for comparative genome analysis of the two Corchorus species.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| |
Keywords: | Jute Corchorus Genetic analysis Simple sequence repeats Genetic diversity |
本文献已被 SpringerLink 等数据库收录! |
|