AFLP技术及其常见问题与解决方案 |
| |
引用本文: | 鞠秀芝,杜胜利,宗兆锋,张桂华,韩毅科,王鸣. AFLP技术及其常见问题与解决方案[J]. 天津农业科学, 2004, 10(4): 6-9 |
| |
作者姓名: | 鞠秀芝 杜胜利 宗兆锋 张桂华 韩毅科 王鸣 |
| |
作者单位: | 1. 西北农林科技大学植保学院,杨凌,712100 2. 天津科润黄瓜研究所,天津,300192 3. 西北农林科技大学园艺学院,杨凌,712100 |
| |
基金项目: | 天津市重大攻关项目(003122011-1) |
| |
摘 要: | 结合工作实践及当前最新研究进展对AFLP分析过程中模板DNA的制备、基因组DNA的酶切与连接、酶切连接产物的预扩增与选择性扩增、扩增产物的变性聚丙烯酰胺凝胶电泳等过程中的常见问题及其解决办法作了简要综述。
|
关 键 词: | AFLP 问题 分析 方案 |
文章编号: | 1006-6500(2004)04-0006-04 |
修稿时间: | 2003-07-15 |
AFLP and Its Common Problems and Solutions |
| |
Abstract: | The common questions in the analyzed procedures of AFLP and its corresponding solutions were briefly summarized based on the working practices and the latest research results. The procedure included preparing DNA samples restriction-ligation of genomic DNA preamplification and selective amplification of products of restriction-ligation and electrophoresis of products of amplification on denaturing polyacrylamide gels. |
| |
Keywords: | AFLP questions analysis solutions |
本文献已被 维普 万方数据 等数据库收录! |
|