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| 云芝菌丝体产SOD的深层发酵培养基的优化 | |
| 引用本文： | 黄志立,黄彦君,张丽君,刘冬,梁世中.云芝菌丝体产SOD的深层发酵培养基的优化[J].中国食用菌,2009,28(4):41-43. |
| 作者姓名： | 黄志立 黄彦君 张丽君 刘冬 梁世中 |
| 作者单位： | 1. 深圳职业技术学院化生学院应用生物技术系,广东,深圳,518055 2. 深圳职业技术学院化生学院应用生物技术系,广东,深圳,518055;华南理工大学生物工程系,广东,广州,510641 3. 华南理工大学生物工程系,广东,广州,510641 |
| 基金项目： | 深圳职业技术学院重点科技开发项目 |
| 摘 要： | 通过设计L16(42)正交实验,确定了可提高云芝菌丝体中SOD活性的深层发酵培养基的最优组分,实验结果表明,培养基各组分对菌丝体生物量的影响顺序依次为黄豆粉>(NH4)2SO4>土豆>葡萄糖>麦麸,而对SOD总活力的影响顺序为(NH4)2SO4>黄豆粉>麦麸>葡萄糖>土豆.综合考虑试验结果及成本因素,确定云芝菌丝体产SOD的最适培养基为马铃薯160 g·L-1、葡萄糖16 g·L-1、麦麸10 g·L-1、黄豆粉18 g·L-1,不添加硫酸铵,pH自然. |
| 关 键 词： | 云芝 菌丝体 SOD 深层发酵 培养基 优化 |
Optimization of Submerged Culture Medium for the Production of Superoxide Dismutase of Coriolus vericolor Mycelia |
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| HUANG Zhi-li,HUANG Yan-jun,ZHANG Li-jun,LIU Dong,LIANG Shi-zhong.Optimization of Submerged Culture Medium for the Production of Superoxide Dismutase of Coriolus vericolor Mycelia[J].Edible Fungi of China,2009,28(4):41-43. | |
| Authors: | HUANG Zhi-li HUANG Yan-jun ZHANG Li-jun LIU Dong LIANG Shi-zhong |
| Institution: | (College of Food Engineering and Biotechnology, South China University of Technology, Guangzhou Guangdong 510640) |
| Abstract: | In this paper, the effects of culture ingredients on the mycelia growth and SOD activity of Coriolus vericolor were observed using an orthogonal layout L16 (45). Results showed that the order of the effects of culture ingredients on the mycelial growth of Coriolus vericolor were soybean powder> (NH4)2SO4>potato>glucose>wheat bran, while the order of the effects of the components on SOD activity were (NH4)2SO4>wheat bran>soybean powder>glucose>potato. The optimal concentration of medium was as follows: 160 g'L-1 potato, 16 g·L-1 glucose, 10 g·L-1 wheat bran, 18 g·L-1 soybean powder and natural pH. |
| Keywords: | SOD Coriolus vericolor Mycelia SOD Optimization Submerged culture Medium ingredients |
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