桑树褐斑病生物学特性研究

通过培养方法分离得到海口林场片区桑树褐斑病病原菌,按照柯赫氏法则采用喷雾法将病原菌接种于桑叶表面进行感染观察,并对病原菌生物学特性进行研究。结果表明,培养基、温度、不同pH值、光照、碳源对菌落的生长影响有所不同。菌落在燕麦培养基中生长最好,最适温度为25℃,最适光照为24 h全光照,最适pH值为11,最适碳源为α-乳糖,菌落的致死温度和时间为55℃水浴10 min。

Biological Characteristics of Mulberry Brown Spot

By using plant tissue culture method, the pathogenic fungi of Mulberry brown was separated spot disease from mulberry fields in The Haikou Forest Farm area, Yunnan Province. According to Koch＇s Rule combined with spraying method, sporangial suspension was inoculate to the leaflet of Mul- berry for studying on the biological characteristics of this fungi. The result showed that different medium, temperature, pH, light, carbon source had influence on colony growth, the optimal media for colony growth was oatmeal medium, the optimum temperature was 25℃ and pH value was 11. It was also found that the best carbon source and nitrogen source for colony grow was α-Galoctaose, colony could grow best under full sunlight, and the fatal temperature and time was above 55℃ for 10min.