| 古银杏雄株的ISSR遗传多样性分析 |
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| 引用本文： | 王国霞,曹福亮,方炎明.古银杏雄株的ISSR遗传多样性分析[J].北京林业大学学报,2010,32(2):39-45. |
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| 作者姓名： | 王国霞 曹福亮 方炎明 |
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| 作者单位： | 南京林业大学森林资源与环境学院 |
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| 基金项目： | “十一五”国家科技支撑计划项目(2006BAD18B0301) |
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| 摘 要： | 应用ISSR分析技术对19个省市的97株古银杏雄株进行遗传多样性分析,并对它们的亲缘关系进行分析鉴定。结果表明:银杏雄株具有丰富的基因多样性和遗传多样性,选用的13个引物共扩增出114条DNA条带,其中多态性DNA条带83条,占72.8%;有效等位基因数为1.8117,基因多样度为0.4374,Shannon信息指数为0.6253;利用UPGMA法对扩增结果进行聚类分析,把97株古银杏雄株分为2大类,一类表现出较强的地理相关性,另一大类结果表明遗传距离与地理位置远近不完全相关。
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| 关 键 词： | 银杏 雄株 ISSR 遗传多样性 |
| 收稿时间： | 1900-01-01 |
Genetic diversity of ancient male ginkgo trees by ISSR analysis |
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| WANG Guo-xia,CAO Fu-liang,FANG Yan-ming.Genetic diversity of ancient male ginkgo trees by ISSR analysis[J].Journal of Beijing Forestry University,2010,32(2):39-45. |
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| Authors: | WANG Guo-xia CAO Fu-liang FANG Yan-ming |
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| Institution: | College of Forest Resources and Environment, Nanjing Forestry University, 210037, P. R. China. |
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| Abstract: | The genetic diversity of 97 ancient male ginkgo trees from 19 provinces was estimated by using ISSR markers to analyze the genetic relation of these individual trees.The results show that male ginkgo trees have high genetic diversity.Thirteen ISSR(inter-simple sequence repeat) primers produced a total of 114 bands,of which 83 were polymorphic.The percentage of polymorphic bands was 72.8% ,the effective number of alleles 1.811 7,Nei's genetic distance was 0.437 4 and Shannon index 0.625 3.UPGMA(unweighted pa... |
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| Keywords: | Ginkgo biloba male tree ISSR genetic diversity |
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