不孕母牛血清抗精子抗体反应与孕酮含量之间的关系

微量滴定板酶免疫法和固相酶染色法分别检测试验组不孕母牛(n=119)和对照组母牛(n=26)包括处女牛、正常空怀母牛和妊娠母牛)的血清孕酮水平和抗精子抗体反应,以研究不孕母牛血清抗精子抗体反应与卵巢功能之间的关系。结果表明:各种原因不孕母牛的血清孕酮含量显著高于处女牛(P>0.05),但与正常空怀母牛和妊娠母牛比较无显著差异(P>0.05)。不孕母牛血清抗精子抗体阳性检出率显著高于对照组(P<0.01),但其抗精子抗体阳性反应血清(n=43)孕酮含量显著低于抗精子抗体阴性反应血清(n=76,P<0.05),而且孕酮含量与抗精子抗体阳性率呈负相关趋势。

THE RELATIONSHIP BETWEEN ANTISPERM ANTIBODIES AND PROGESTERONE IN THE SERUM OF INFERTILE DAIRY COWS

By the means of EIA and Solid Enzyme Staining Assay ( SESA ) , the level of progesterone ( P ) and reaction of antisperm antibodies ( As-Ab ) in the serum of infertile dairy cows ( experimental group, n = 119) and heifers, normal barren and pregnant cows ( control group, n = 26 ) were detected to study the relationship between AsAb of the serum and ovarian function of infertile cows.The results showed that the content of P in the serum of all patterns of infertile cows was significantly higher than that of heifers ( P<0.05 ) , although there was no significant difference as compared with barren and pregnant cows(P> 0.05 ) . The positive reaction rate of AsAb in the serum of infertile cows was obviously higher than that of the control group ( P<0.01 ) , but the P level of serum reacted positive in AsAb ( n = 43 ) was lower than that of serum reacted negative in AsAb (n = 76, P<0.05 ) , and there was a tendence to negative correlation between the level of P and positive reaction rate of AsAb.