中华蜜蜂卵冷冻贮存技术

以中华蜜蜂（Ａｐｉｓｃｅｒａｎａｃｅｒａｎａ）为材料，用玻璃化法进行了反复多次的卵冷冻试验．结果表明：卵冷冻的最适剂型为颗粒冷冻，最适的冷冻保护剂为体积分数为０．２５甘油＋０．２５乙二醇＋１．０ｍｏｌＬ－１蔗糖，预冻的时间为２ｍｉｎ，离液面的高度为２ｃｍ，脱除冷冻保护剂用的稀释液以６．５ｇＬ－１的生理盐水为最好．现已初步获得成功：冷冻后的中华蜜蜂卵能正常孵化为幼虫，但其孵化率较低（２５．０％～３７．５％），育王时尚不能被工蜂所接受．对此，今后仍需进一步试验．

Frozen preservation techniques of Chinese honeybee eggs

Chinese honeybee(Apis cerana cerana) eggs were frozen by vitrification method in liquid nitrogen. Results showed that the most suitable form for egg freezing was granular sphere, the most suitable cryoprotectant was 0.25 glycerin+0.25ethylene glycol+1.0 mol·L-1 sucrose (V/V), the most suitable prefreezing time was 2 min, and the height above the liquid nitrogen surface was 2 cm, the best diluent was 6.5 g·L-1 physiological saline. It was successful primarily to have made frozen eggs hatch normally. The further experiment would be made because the hatchability of post thawed eggs was low(25.0%～37.5%), the larva was uncapable of being received by workers when it was grafted to the queen cell cup for queen breeding.