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| 猪Myl1基因启动子克隆及多态性研究 | |
| 引用本文： | 凌飞,李加琪,钟万福,杜红丽,陈瑶生. 猪Myl1基因启动子克隆及多态性研究[J]. 广东农业科学, 2009, 0(6): 127-130 |
| 作者姓名： | 凌飞 李加琪 钟万福 杜红丽 陈瑶生 |
| 作者单位： | 1. 华南理工大学生物与工程学院,广东,广州,510641;华南农业大学动物科学学院,广东,广州,510642 2. 华南农业大学动物科学学院,广东,广州,510642 3. 华南理工大学生物与工程学院,广东,广州,510641 4. 中山大学生命科学学院,广东,广州,510275 |
| 基金项目： | "973"项目 |
| 摘 要： | 肌球蛋白轻链1基因(Myl1)编码的不同剪切体(主要是MLC1f和MLC3f)与哺乳动物骨骼肌肌纤维类型相关.通过猪BAC文库筛选及引物步行法测序获得Myl1基因的启动子序列,采用PCR-RFLP技术分析蓝塘猪、大花白猪、大白猪、长白猪和杜洛克猪5个品种Myl1基因启动子区的多态性.结果表明,Myl1基因-678C/T位点在蓝塘和大花白等本地猪种中多态性丰富,而在杜洛克、长白和大白等外来猪种中几乎只有CC基因型. |
| 关 键 词： | 肌球蛋白轻链1基因 猪BAC文库 启动子 单核苷酸多态性 |
Molecular cloning and polymorphism of the pig Myl1 promoter |
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| LING Fei,LI Jia-qi,ZHONG Wan-fu,DU Hong-li,CHEN Yao-sheng. Molecular cloning and polymorphism of the pig Myl1 promoter[J]. Guangdong Agricultural Sciences, 2009, 0(6): 127-130 | |
| Authors: | LING Fei LI Jia-qi ZHONG Wan-fu DU Hong-li CHEN Yao-sheng |
| Affiliation: | LING Fei1,2,LI Jia-qi2,ZHONG Wan-fu2,DU Hong-li1,CHEN Yao-sheng3(1.School of Biological Science and Engineering,South China University of Technology,Guangzhou 510006,China,2.College of Animal Science,South China Agricultural University,Guangzhou 510642,3.School of Life Science,Sun Yat-Sen University,China) |
| Abstract: | Distinctive alternative variants of Myl1(MLC1f and MLC3f) were found to be associated with the skeletal muscle fiber type of mammalian.The 5'-flanking region of the Myl1 gene was isolated from a pig BAC library and was sequenced by primer-walking method,which was used to analyse polymorphism of breeds(40 LanTang pigs,40 DaHuaBai pigs,80 landrace pigs,80 large white pigs and 50 duroc pigs) with PCR-RFLP.The results showed that the SNP of-678C/T was identified in the Myl1 promoter.Distribution of genotype in ... |
| Keywords: | Myl1 gene pig BAC library promoter SNP |
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