草鱼出血病细胞培养灭活疫苗的研究——疫苗株的免疫原性及其有效免疫剂量的比较

本文比较了疫苗株FR—854和FR—836—w的免疫原性,及其对三寸左右草鱼种的有效免疫剂量。疫苗株FR—854和FR—836—w均有较强的免疫原性,其保护力可达70%以上。但试验结果初步证明,FR—854疫苗比FR—836—w疫苗免疫原性更强。经统计分析,FR—854疫苗的免疫保护力可达88.9±12.0（6）%,血清中和抗体效价为160.5±58.9（6）;而FR-838-W疫苗分别只有71.3±14.2（6）%、88.3±26.2（6）;二者有显著的差异（0.05>P>0.01）。注射免疫三寸左右的草鱼种,FR—854的免疫剂量在3—5×10^4.5TCID0/尾左右,FR—836—w在3—5×107.5TCID₅₀/尾左右,其免疫保护力可达80%左右,同时我们测得ER—854的半数免疫量为10^5.1TCID₅₀/ml。

INACTIVE VACCINE FOR HEMORRHAGE OF GRASS CARP:COMPARISON OF IMMUNOGENICITY AND IMMUNIZING DOSE BETWEEN TWO STRAINS

Vaccine FR-854 and FR-836-w have high immungenicity and theirPRP (percentage relative protection) are both more than 70%. However, it ispreliminarily demonstrated that strain FR-854 has much higher immunogenicitythan strain FR-836-w by means of measuring the PRP and ANT (antiserum neu-tralization titre) of vaccinated grass carp with them. It hes been obtained that PRPand ANT are 88.9±12.0(6)% and 160.5±58.9 (6) respectively for FR-854, while 71.3±14.2(6)% and 88.3±26.2 (6) for FR-836-w. Also, the effective immunizingdoses to 1⁺or 0⁺ grass carp, 3-5×10^(4.5)TCID₍₅₀₎/fish for FR-854 and 3-5×10^(7.5)TCID⁽⁵⁰⁾/fish for FR-836-w, are all determined. The results show that immunopro-phylaris to the hemorrhage of grass carp with this inactive vaccine is in prospect.