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| 小麦品种的抗赤霉病性与PGIP含量和分布的关系 | |
| 引用本文： | 阮期平,周立,李建吾,刘勇,曹阳. 小麦品种的抗赤霉病性与PGIP含量和分布的关系[J]. 植物保护学报, 2002, 29(2): 101-104 |
| 作者姓名： | 阮期平 周立 李建吾 刘勇 曹阳 |
| 作者单位： | 1. 四川绵阳师范高等专科学校生物系,绵阳,621000 2. 四川大学生物科学与工程学学院,成都,610064 3. 四川省农业科学院植物保护研究所,成都,610066 |
| 基金项目： | 国家自然科学基金(39880030) |
| 摘 要： | 研究了小麦品种的抗赤霉病性与多聚半乳糖醛酸酶抑制蛋白(PGIP)含量和分布的关系。斑点免疫结合测定(Dot-immunobinding assay)和蛋白质印迹分析(Western blotting analyze)结果显示,SW89-2589品种的PGIP含量高于89-106-2-9-24、80-8及3625品种;免疫组织化学定位表明,SW89-2589品种PGIP分布比80-8品种更为广泛。由此可见,小麦品种对赤霉病的抗性愈强,PGIP含量愈高、分布愈广。此研究结果为选育小麦抗性品种提供了一种新的鉴定方法。 |
| 关 键 词： | 小麦 赤霉病 PGIP 品种抗性 |
The relationships between the scab-resistance and PGIP of wheat varieties |
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| Ruan Qiping,Zhou Li,Li Jianwu,Liu Yong and Cao Yang. The relationships between the scab-resistance and PGIP of wheat varieties[J]. Acta Phytophylacica Sinica, 2002, 29(2): 101-104 | |
| Authors: | Ruan Qiping Zhou Li Li Jianwu Liu Yong Cao Yang |
| Affiliation: | Academy of Life-science and Engineering, Sichuan University, Chengdu 610064,Academy of Life-science and Engineering, Sichuan University, Chengdu 610064,Academy of Life-science and Engineering, Sichuan University, Chengdu 610064,Institute of Plant Protection, Sichuan Academy of Agricultural Science, Chengdu 610066 and Institute of Plant Protection, Sichuan Academy of Agricultural Science, Chengdu 610066 |
| Abstract: | The relationships between scab-resistance and content or distribution of PGIP were investigated in wheat varieties. Dot-immunobinding assay and western blotting analysis showed that the level of PGIP in variety SW89-2589 was more than that in 89-106-2-9-2-4, 80-8 and 3625. Im-munocytochemistry also found that the distribution of PGIP in variety SW89-2589 was wider than that in 80-8. These indicated that the level and the distribution of PGIP in wheat was closely associated with resistance of wheat varieties to Giberalla zeae . All results suggest that the analysis of PGIP can be developed as a new method for identifying the resistance of wheat varieties to G. zeae. |
| Keywords: | wheat Gibberella zeae PGIP variety resistance |
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