In vitro fermentation of lactulose-derived oligosaccharides by mixed fecal microbiota |
| |
Authors: | Cardelle-Cobas Alejandra Olano Agustín Corzo Nieves Villamiel Mar Collins Michelle Kolida Sofia Rastall Robert A |
| |
Institution: | Instituto de Investigación en Ciencias de la Investigación CIAL (CSIC-UAM ), C/Nicolás Cabrera 9, Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain. |
| |
Abstract: | Fermentation properties of oligosaccharides derived from lactulose (OsLu) and lactose (GOS) have been assessed in pH-controlled anaerobic batch cultures using lactulose and Vivinal-GOS as reference carbohydrates. Changes in gut bacterial populations and their metabolic activities were monitored over 24 h by fluorescent in situ hybridization (FISH) and by measurement of short-chain fatty acid (SCFA) production. Lactulose-derived oligosaccharides were selectively fermented by Bifidobacterium and lactic acid bacterial populations producing higher SCFA concentrations compared to GOS. The highest total SCFA production was from Vivinal-GOS > lactulose > OsLu > GOS. Longer incubation periods produced a selective fermentation of OsLu when they were used as a carbon source reaching the highest selective index scores. The new oligosaccharides may constitute a good alternative to lactulose, and they could belong to a new generation of prebiotics to be used as a functional ingredient for improving the composition of gut microflora. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|