Genetic relationships among 23 Ficus accessions using inter-simple sequence repeat markers |
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Authors: | Gyana Ranjan Rout Subhashree Aparajita |
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Institution: | 1. Plant Biotechnology Division, Regional Plant Resource Centre, Bhubaneswar, 751015, Orissa, India
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Abstract: | Ficus genus is widely distributed in all the climatic stages and great diversity. The exploration of genetic diversity is a pre-requisite
for genome organization in the wild species and the related domesticated ones. Inter-Simple Sequence Repeat (ISSR) markers
were used to assess the identification of 23 important Ficus accessions and determination of the genetic relationships among these accessions. Out of 21 ISSR primers tested, five primers
produced 116 detectable fragments, of which 106 (91.3%) were polymorphic across the accessions. Each of the five primers produced
a fingerprint profile unique to each of the accessions studied, and thus could be solely used for their identification. Thirteen
unique bands specific to nine species were detected. These may be converted into species-specific probes for identification
purposes. Genetic relationships among these accessions were evaluated by generating a similarity matrix based on the Dice
coefficient and the Unweighted Pair Group Method with Arithmetic Average (UPGMA) dendogram. The results showed a clear cut
separation of the 23 Ficus accessions and were in broad agreement with the morphology. Both molecular and morphological markers will be useful for preservation
of the Ficus germplasm. |
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