| 亚胺环己酮对猪卵母细胞人工孤雌激活作用的研究 |
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| 引用本文： | 李光鹏,孟庆刚,魏鹏,李子义,孙兴参,谭景和. 亚胺环己酮对猪卵母细胞人工孤雌激活作用的研究[J]. 畜牧兽医学报, 2001, 32(5): 416-420 |
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| 作者姓名： | 李光鹏 孟庆刚 魏鹏 李子义 孙兴参 谭景和 |
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| 作者单位： | 东北农业大学生物工程系 |
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| 基金项目： | 国家教委跨世纪优秀人才计划基金(1994)和黑龙江省杰出青年科学基金(1995)资助项目 |
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| 摘 要： | 本文以体外成熟的猪卵母细胞为材料，以乙醇、电脉冲和氯化锶为人工刺激条件，研究了蛋白质合成抑制剂－亚胺环己酮（CHX）对猪卵母细胞的孤雌激活效果的影响。结果表明，乙醇、电脉冲和SrCl2均可使猪母细胞激活，而电脉冲的活效果最好。当分别与CHX联合使用时，激活率显著高于单独的乙醇、电脉冲或SrCl2刺激。说明，CHX与乙醇、电脉冲及SrCl2联合使用对猪IVM卵母细胞激活具有显著的协同促进作用。
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| 关 键 词： | 亚胺环己酮 孤雌激活 卵母细胞 猪 细胞核移植 |
| 文章编号： | 0366-6964(2001)05-0416-05 |
ARTIFICIAL ACTIVATION OF POR CINE OOCYTES MATURED IN VITRO |
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| LI Guang peng,MENG Qing gang,WEI Peng,LI Zi yi,SUN Xing shen,TAN Jing he. ARTIFICIAL ACTIVATION OF POR CINE OOCYTES MATURED IN VITRO[J]. Chinese Journal of Animal and Veterinary Sciences, 2001, 32(5): 416-420 |
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| Authors: | LI Guang peng MENG Qing gang WEI Peng LI Zi yi SUN Xing shen TAN Jing he |
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| Abstract: | This study was designed to evaluate the effects of cycloheximide (CHX),a protein synthesis inhibitor,on artificial activation of in vitro matured (IVM) porcine oocytes with alcohol,electric pulse or strontium cholide (SrCl 2),respectively.The results showed that alcohol,electric pulse or SrCl 2 could activate the porcine oocytes which were cultured in modified TCM 199 media for 44 48 h.When the oocytes were primed with alcohol,electric pulse or SrCl 2 and then cultured in CHX contained TCM 199 media for 20 h,the activation rates were significantly higher than that stimulated with alcohol,electric pulse or SrCl 2 alone.In conclusion,alcohol,electric pulse and strontium chloride acted synergistically with CHX in activating the porcine IVM oocytes. |
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| Keywords: | Cycloheximide (CHX) Parthenogenetic activation Oocytes Porcine |
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