水稻黄单胞杆菌水稻变种rpfA基因与毒性相关

水稻黄单胞杆菌水稻变种产生两种顺乌头酸酶（XooAcanA和XooAcanB)。编码其中的XooAcanA的rpfA基因已被克隆在重组质粒pGXN3000中。本研究通过转座子诱变pGXN3000及标记置换方法，构建了rpfA突变体T3A01。虽然T3A01丧失了合成顺乌头酸酶XooAcanA的能力，但仍然能够合成顺顺乌头酸酶XooAcanB，植株实验结果表明，该突变体的生长速率与野生型相似。但是引起的症状与野生型相比明显减弱，说明水稻黄单胞杆菌水稻变种的rpfA基因与毒性相关。

The rpfA gene ofXanthomonas oryzae pv.oryzae involved in the pathogenicity

It was demonstrated that Xanthomonas oryzae pv. oryzae (Xoo)produced two aconitase (designated XooAcanA and XooAcanB).The rpfA gene of Xoo encoding XooAcanA had been cloned in a recombinant plasmid pGXN3000 previously.On the basis of transposon Tn5-B20 insertion mutagenesi s and marker exchange,a rpfA mutant strain T3A01 was obtained.T3A01 was sho wn to lack the aconitase XooAcanA activity but retained the aconitase XooAcanB a c tivity.It was shown that T3A01 could reduce pathogenicity when bacteria were in troduced into the rice plant,although it grew at similar rates to the wild type strain 13751,suggesting that the rpfA gene of Xoo was involved in the pathogenicity.