Analysis of the ozone effect on soybean in the Mediterranean region: II. The consequences on growth, yield and water use efficiency

Soybean plants were subjected, during their growing seasons, to well-watered and water-stressed conditions, and three levels of ozone concentration (zero, low and high) in open top chambers (OTCs). At the end of the soybean growing season accumulated AOT40 values were zero, 3400 and 9000 ppb h for the filtered (control), low and high levels of ozone concentration, respectively.

In well-watered conditions, an increase in ozone concentration led to a reduction in leaf area, dry matter and reproductive organs. Whereas, an increase in ozone had no effect on plants in water-stressed conditions. At a high level of ozone concentration, there was a 47% reduction in yield and a 25% reduction in WUE in comparison with the control treatment. The reduction in yield was due to a lower number of pods per plant and 1000-grain weight. Despite changes in the grain yield, the yield quality was not altered by ozone.

During the 3-year study, AOT40 was significantly correlated with the leaf area and the final above-ground dry matter. The latter was less sensitive to ozone than leaf area. These results were reliable and would be useful in soybean yield-prediction models.

Finally, the conclusion highlights the reliability of the approach adopted, which was to make observations on various time scales (hourly, daily and entire crop cycle).