Responses of peripheral blood mononuclear cells in calves infected with Theileria sergenti |
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| Authors: | Y Yasutomi M Onuma H Asaoka Y Kawakami |
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| Affiliation: | Department of Veterinary Microbiology, Rakuno Gakuen University, Hokkaido, Japan. |
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| Abstract: | The role of peripheral blood mononuclear cell (PBMC) in Theileria sergenti-infected calves was studied by various in vitro assay systems. Proliferation of T cells in mixed lymphocyte protozoa culture (MLPC) increased with parasitemia, and the addition of monoclonal antibodies against T. sergenti merozoites in this MLPC enhanced the response. However, the addition of antibody-positive autologous serum resulted in the suppression of the response. Cell-mediated cytotoxicity of PBMC increased after peak parasitemia. This cytotoxicity increased on co-cultivation of PBMC with T. sergenti merozoites, but the addition of autologous serum suppressed the response. |
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