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| PCR结合DGGE技术快速鉴定橘小实蝇及其近缘种 | |
| 引用本文： | 詹开瑞,赵士熙,陈宇航,任大明. PCR结合DGGE技术快速鉴定橘小实蝇及其近缘种[J]. 福建农林大学学报(自然科学版), 2007, 36(3): 274-278 |
| 作者姓名： | 詹开瑞 赵士熙 陈宇航 任大明 |
| 作者单位： | 1. 福建农林大学植物保护学院,福建,福州,350002;福建出入境检验检疫局,福建,福州,350013 2. 福建农林大学植物保护学院,福建,福州,350002 3. 复旦大学生命科学学院遗传所,上海,200433 |
| 摘 要： | 首次采用PCR结合变性梯度凝胶电泳(DGGE)技术对橘小实蝇及其近缘种的线粒体COⅡ和16S rDNA基因序列进行分析.结果表明,通过DGGE可以区分实蝇属不同种类的COⅡ和16S rDNA基因序列. |
| 关 键 词： | 橘小实蝇 检疫 PCR-DGGE 种类鉴定 |
| 文章编号： | 1671-5470（2007）03-0274-05 |
| 修稿时间： | 2006-04-102007-04-25 |
Rapid identification of 6 species of fruit fly (Diptera: Tephritidae) by PCR-DGGE |
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| ZHAN Kai-rui,ZHAO Shi-xi,CHEN Yu-hang,REN Da-ming. Rapid identification of 6 species of fruit fly (Diptera: Tephritidae) by PCR-DGGE[J]. Journal of Fujian Agricultural and Forestry University, 2007, 36(3): 274-278 | |
| Authors: | ZHAN Kai-rui ZHAO Shi-xi CHEN Yu-hang REN Da-ming |
| Affiliation: | 1. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2. Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou, Fujian 350013, China; 3. College of Life Sciences, Fudan University, Shanghai 200433, China |
| Abstract: | Bactrocera dorsalis(Hendel) and the other five Bactrocera species(B.latifrons,B.tau,B.lateriataenia,B.scutellata,B.cucurbitae) were identified by PCR-DGGE method based on the gene analysis of mtDNA COⅡ and 16S rDNA.The result showed PCR-DGGE was a rapid and specific technique for pest species identification in plant quarantine. |
| Keywords: | Bactrocera dorsalis(Hendel) quarantine PCR-DGGE(denaturing gradient gel electrophoresis) species identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |