~(60)Coγ射线对小豆种子辐射处理效应的研究

本试验研究结果表明 :60 Coγ射线辐照小豆种子的半致死剂量平均为 3 89Gy ,且M1生长初期叶片性状有明显的叶面缺刻、叶色缺绿、叶形不对称、叶缘卷曲、花叶、黄叶等致变现象 ,各种畸型叶出现频率与辐照剂量大小有关 ;M1根尖细胞中有多种染色体畸变类型 ,以染色体桥和断片频率较高 ,辐照剂量在 40 0Gy以上时根尖细胞异常率达 1 0 %以上 ;植株花粉育性与辐照剂量呈极显著负相关 ,京农 2号植株花粉育性达 5 0 %时的辐照剂量为 3 78Gy。根据M1出苗率、成活株率、根尖细胞异常率、叶片致畸率、植株花粉育性、植株农艺性状受抑程度及M2 植株性状的变异等综合分析 ,60 Coγ射线辐照小豆种子的较适宜剂量为 40 0Gy

A STUDY ON THE RADIATIVE MUTATION ON THE SEEDS OF ADZUKI BEAN BY ~(60)Co γ RAY

The seeds of Adzuki bean were irradiated by 60 Co γ ray of 0～700Gy.The results showed that:(1)The sub|lethal dose(LD 50 )was 389Gy and the early seedling leaf at M-1 showed incised,chlorotic,yellow,mosaicand curly edge.The absorbed dose was related with the rate of misshaped leaf.Chromosomal aberration, chromosome bridge and fragment of M-1 in the tip were observed.If the seed was irradiated with 400Gy,the rate of abnormal cell at the root was more than 10%.The relationship between pollen fertility and irradiation dose was negative correlated.When the irradiation dose was 387 Gy,the pollen fertility was 50% in the seeds of Adzuki bean.(2)The optimum irradiation dose for the seed of Adzuki bean is 400Gy in the M-1 and M-2.