牛凸隆病毒HE基因分子特征分析

本研究旨在分析我国牛凸隆病毒(bovine torovirus,BToV)的HE基因分子特征.采用RT-PCR方法检测辽宁、河南、四川的牛腹泻样本中BToV,阳性样本扩增完整HE基因.结果显示:从94份腹泻粪便中检出10份BToV阳性,平均阳性率约为10.64％.从10份阳性样本中扩增得到15条HE基因,其中,9条为基...

Molecular Characteristics Analysis of HE Genes of Bovine Torovirus

The purpose of this study was to characterize the molecular characteristics of the HE genes of bovine torovirus (BToV). An RT-PCR assay was used to detect BToV from 94 diarrheic fecal samples collected in Liaoning, Henan, and Sichuan provinces and full-length hemagglutinin-esterase (HE) genes were further amplified from the positive samples. Approximate 10.64% (10/94) samples were detected as BToV positive, and 15 full-length HE genes were sequenced from 10 positive samples, including 9 genotype Ⅱ strains and 6 genotype Ⅲ strains. Among the 10 positive samples, 5 samples were co-infected with genotype Ⅱ and Ⅲ strains, 1 sample was infected with genotype Ⅲ only and 4 samples were infected with genotype Ⅱ strains only. Compared with other available genotype Ⅲ BToV HE genes in the GenBank database, 4 unique amino acid variations were found in the HE gene, including 2 variations in the lectin domain which may affect HE receptor binding. Recombination analysis showed that BToV genotype Ⅲ strains may be derived from the recombination of genotype Ⅱand genotype Ⅰstrains in the HE gene. This study is the first confirmation of genotype Ⅲ BToV in China and the co-infection of genotype Ⅱ and Ⅲ strains. These findings contributed to the diagnosis and control of bovine diarrhea in China and enhance the current understanding of the genetic evolution of BToV.