右美托咪定对氯胺酮致发育期大鼠神经损伤的影响

本研究旨在探讨右美托咪定干预氯胺酮致发育期大鼠神经损伤的影响及其可能的机制.7日龄SD大鼠随机分为对照组、氯胺酮组(氯胺酮20 mg·kg-1腹腔注射,每1.5h注射1次,共5次)、右美托咪定组(右美托咪定腹腔注射15 μg·kg-1)和氯胺酮+右美托咪定组(氯胺酮注射前30 min,腹腔注射15 tg·kg-1右美托...

Effects of Dexmedetomidine on Ketamine Induced Nerve Injury in Developing Rats

The purpose of this study was to investigate the effect of dexmedetomidine on ketamine induced nerve injury in developing rats and its possible mechanism. Seven-day-old SD rats were randomly divided into control groups; ketamine group (20 mg·kg^-1 ketamine, i.p. once every 1.5 hours, 5 times); dexmedetomidine group (15 μg·kg^-1 dexmedetomidine, i.p); ketamine + dexmedetomidine group (30 min before ketamine injection, 15 μg·kg^-1 dexmedetomidine, i.p.). Ninety minutes after the last administration, the brain tissue was taken and fixed for Nissl staining; the contents of CAT, GSH, MDA, IL-1β and IL-18 in the hippocampus and cortical tissue were measured. The results of Nissl staining showed that pre-administration of dexmedetomidine could alleviate the loss of neurons in hippocampal CA1, CA3, and cortex caused by ketamine compared with the control group. Dexmedetomidine pretreatment could also significantly reduce (P<0.05) the levels of MDA, IL-1β and IL-18 in the hippocampus and cortex, and significantly increase (P<0.05) the content of CAT and GSH. The results of the study show that dexmedetomidine pretreatment can effectively reduce hippocampal and cortical MDA levels, increase CAT and GSH content, and inhibit the secretion of IL-1β and IL-18, and exert neuroprotection when ketamine-induced neurological damage in developmental rats.