纳米硒对氟化钠诱导小鼠肝组织细胞凋亡的影响

旨在探究纳米硒对氟化钠诱导肝细胞凋亡的影响,40只28日龄昆明小鼠(25 g±2 g)适应性饲养7 d后,随机分为对照组(Control,生理盐水)、氟化钠组(NaF,24 mg·kg-1)、纳米硒组(Nano-Se,1 mg·kg-1)、纳米硒治疗组(NaF+Nano-Se,24 mg·kg-1 NaF+1 mg·k...

Effect of Nano-selenium on the Apoptosis Induced by Fluoride in Hepatocytes

In order to explore the effect of nano-selenium on the apoptosis of hepatocytes induced by fluoride, A total of 40 Kunming mice were divided into 4 groups (each group contents 10 mices) after 7 days of adaptive feeding, which were administered with normal saline, 24 mg·kg^-1 NaF, 1 mg·kg^-1 nano-selenium and the same dose of NaF (24 mg·kg^-1 NaF) + nano-selenium (1 mg·kg^-1). The entire experiment lasted for 28 days, during which all the mice had free drinking and eating. The pathological damage of hepatocyte was evaluated by HE staining, while Tunel staining was used to evaluate the apoptosis level of hepatocytes, and the expression level of apoptosis-related genes and proteins were detected by immunofluorescence, Western blot, and RT-qPCR. The results showed that nano-selenium effectively alleviates the swelling of liver cells, vacuolar degeneration and nuclear fragmentation caused by fluoride treatment, and down-regulates the expression of genes related to apoptosis, such as Bax, Caspase3/9 and protein P53, Caspase3, elevated Bcl-2/Bax expression level. Tunel staining results showed that fluoride treatment significantly increased the rate of cell apoptosis (P<0.01), while nano-selenium can effectively reduce the incidence of cell apoptosis(P<0.05). The expression level of Cyt-C in the fluoride group was significantly higher than that in the control group (P<0.05), while the expression in the nano-selenium treatment group showed a downward trend. In conclusion, nano-selenium can effectively alleviate fluoride induced hepatocyte apoptosis by regulating the expression of apoptosis-related factors.