小鼠营养性肥胖模型的建立

目的]建立与人类肥胖最为接近的高脂饲料所致单纯性肥胖模型,为肥胖理论研究奠定基础。方法]选用ICR和KM 2个品系的小鼠,雌雄各半,按体重随机分为高脂饲料试验组和普通饲料对照组,测定小鼠体重L、ee’s指数、脂肪湿重、同视野脂肪细胞数量及血液指标。结果]ICR雌性小鼠各项指标与对照组相比均有统计学差异(P<0.01或P<0.05)。结论]不同品系的小鼠对高脂饲料的要求不同,相同品系不同性别的小鼠也需要不同的高脂饲料诱导营养性肥胖模型。

Construction of Mouse Nutritional Obesity Model

Objective] The aim of this study is to construct the model for simple obesity induced by high-fat diet,which is closest to human obesity,laying a foundation for the studies of obesity related theories.Method] ICR and KM mice,half male and half female,were randomly divided into high-fat diet experimental group and normal diet control group based on body weight,and certain days later,body weight,Lee's index,wet weight of adipose tissue,quantity of adipose cell in the same visual field and blood indices were measured.Result] All indices mentioned above of the female ICR mouse had significant statistical differences with those of the control group(P<0.01 or P<0.05).Conclusion] To construct mouse nutritional obesity model successfully,different high-fat diets were required by different lines as well as different sexes in the same line.