| 乌鸡传染性法氏囊病毒VP2基因的克隆与序列分析 |
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| 引用本文： | 张春杰,程相朝,李银聚. 乌鸡传染性法氏囊病毒VP2基因的克隆与序列分析[J]. 西北农林科技大学学报(自然科学版), 2004, 32(3): 33-36 |
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| 作者姓名： | 张春杰 程相朝 李银聚 |
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| 作者单位： | 河南科技大学,动物科技学院,河南,洛阳,471003 |
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| 基金项目： | 河南省骨干教师资助项目(2003056) |
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| 摘 要： | 应用RT-PCR法,对分离于当地典型发病乌鸡群的IBDV(WJ株)进行了VP2基因的克隆与序列分析,并和相关毒株进行了比较。结果表明,WJ株与标准血清 型STC株的核苷酸和氨基酸的同源性只有91.39%和92.74%,而与超强毒株UK661和从当地鸡群中分离的超强毒株HN01株的核苷酸和氨基酸的同源性则较高,其中,与其核苷酸同源性分别为95.43%和95.87%,与其氨基酸同源性均为96.77%。且该毒株的VP2基因序列完全具备超强毒株的主要特征。由此说明,分离于当地发病乌鸡群的IBDV为超强毒株,并和当地流行的鸡IBD超强毒株有较高的同源性,但也有明显的差异。
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| 关 键 词： | 乌鸡 IBDV VP2基因 克隆 序列分析 |
| 文章编号： | 1671-9387(2004)03-0033-04 |
| 收稿时间： | 2003-07-22 |
| 修稿时间： | 2003-07-22 |
Cloning and sequence analysis of the VP2 gene of IBDV isolated from dark-bone chicken |
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| ZHANG Chun-jie,CHENG Xiang-chao,LI Yin-ju,WU Ting-cai,WANG Shu-fang. Cloning and sequence analysis of the VP2 gene of IBDV isolated from dark-bone chicken[J]. Journal of Northwest A&F University（Natural Science Edition）, 2004, 32(3): 33-36 |
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| Authors: | ZHANG Chun-jie CHENG Xiang-chao LI Yin-ju WU Ting-cai WANG Shu-fang |
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| Affiliation: | (College of Animal Science and Technology,Henan University of Science and Technology,Luoyang,Henan 471003,China) |
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| Abstract: | The VP2 gene of IBDV (WJ strain) isolated from dark-bone chicken were amplified by RT-PCR and cloned into pMD18-T vector to analysed sequence.Sequence comparison with standard I sterotype IBDV (STC),the homology of the nucleotide and amino acid were only 91.39% and 92.74%,separately;But comparison with very-virulent IBDV (vv IBDV) UK661 and HN01,the homology of nucleotide were 95.43% and 95.87%,96.77%;the homology of amino acid all were 96.77%.Moreover,the VP2 gene sequence of WJ strain possessed completely the characteristics of vv IBDV.The results showed that WJ strain was a very-virulent IBDV. |
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| Keywords: | dark-bone chicken IBDV VP2 gene cloning sequence analysis |
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