Comparison of enzyme-linked immunosorbent assay and immunofluorescence test for determination of anti-Encephalitozoon cuniculi antibodies in sera from rabbits with different clinical and histopathological presentations |
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| Institution: | 1. University of Miami, Division of Comparative Pathology, Department of Pathology & Laboratory Medicine, Miller School of Medicine, Miami, FL, USA;2. University of Miami, Department of Epidemiology and Public Health, Miller School of Medicine, Miami, FL, USA;3. Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria;4. University Hospital for Small Animals, Internal Medicine of Small Animals, Department for Companion Animals and Horses, University of Veterinary Medicine, Vienna, Austria;1. University of Illinois, Department of Veterinary Clinical Medicine, Urbana, IL, USA;2. Mystic Aquarium, Mystic, CT, USA;3. Oxbow Animal Health, Murdock, NE, USA;4. Scovill Zoo, Decatur, IL, USA;5. Louisiana State University, Department of Veterinary Clinical Sciences, Baton Rouge, LA, USA.;1. William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California-Davis, Davis, CA, USA;2. Department of Veterinary Clinical Sciences, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, USA;3. Departments of Pathology College of Veterinary Medicine, University of Georgia, Athens, GA, USA.;4. Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.;5. Department of Small Animal Medicine And Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.;1. FauneVET, Nantes, France;2. Clinique Vétérinaire des Rochettes, Montaigu, France;3. Centre Hospitalier Vétérinaire Atlantia, Nantes, France;1. Clinique Vétérinaire Benjamin Franklin, Brech, France;2. Clinique Vétérinaire Alliance, Bordeaux, France;1. Department of Companion Animals and Horses, University of Veterinary Medicine Vienna, Vienna, Austria;2. Department for Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria;1. Avian & Exotic Animal Clinic, Indianapolis IN, USA;2. Eiken Chemical Co., Taito-ku, Tokyo, Japan;3. Department of Epidemiology and Public Health, University of Miami Miller School of Medicine, Miami, FL, USA;4. Division of Comparative Pathology, Department of Pathology, University of Miami Miller School of Medicine, Miami, FL, USA |
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| Abstract: | Encephalitozoon cuniculi is a microsporidian that infects rabbits resulting in a varied pathology of the nervous, renal, and ocular tissues. Serology based studies using different methods have been conducted indicating a high seroprevalence worldwide in pet rabbits. While qualitative results are not especially helpful in the diagnosis of this infection, quantitative titers can have increased predictive value. In the current study, results of enzyme-linked immunosorbent assay (ELISA) and immunofluorescence test methods were compared using banked samples from rabbits with confirmed infection and clinically normal rabbits. Pearson's analysis found good and significant correlation (R = 0.56, P = 0.0002). Bland Altman analysis demonstrated the methods were similar but not equivalent. The sensitivity ranged from 0.79 to 0.84 and the specificity was 1.00 for both assays. Anti-E. cuniculi IgM titers were also determined by ELISA to be an independent measure from IgG (R = 0.31, P = 0.06) with a sensitivity of 0.24 and specificity of 1.00. In total, these results confirm an agreement in the detection of IgG antibody by two different methods conducted at laboratories in the United States and Austria that are involved in research and clinical diagnostic testing of this agent. |
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