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Effects of cryopreservation on the mitochondrial bioenergetics of bovine sperm |
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| Authors: | Cristina Algieri Olga Blanco-Prieto Marc Llavanera Marc Yeste Marcella Spinaci Gaetano Mari Diego Bucci Salvatore Nesci |
| Institution: | 1. Department of Veterinary Medical Sciences, Alma Mater Studiorum–University of Bologna, Bologna, Italy;2. Department of Veterinary Medical Sciences, Alma Mater Studiorum–University of Bologna, Bologna, Italy
Biotechnology of Animal and Human Reproduction (TechnoSperm), Unit of Cell Biology, Department of Biology, Faculty of Sciences, Institute of Food and Agricultural Technology, University of Girona, Girona, Spain;3. Biotechnology of Animal and Human Reproduction (TechnoSperm), Unit of Cell Biology, Department of Biology, Faculty of Sciences, Institute of Food and Agricultural Technology, University of Girona, Girona, Spain;4. Department of Veterinary Medical Sciences, Alma Mater Studiorum–University of Bologna, Bologna, Italy National Institute for Artificial Insemination—AUB INFA, University of Bologna, Bologna, Spain |
| Abstract: | This study evaluated the bioenergetic map of mitochondria metabolism in cryopreserved bovine sperm. The detected oligomycin-sensitive basal respiration supported ATP production; frozen–thawed spermatozoa were found to have a coupling efficiency higher than 0.80. Cell respiration, however, was not stimulated by the protonophoric action of FCCP, as its titration with 1, 2, 4 and 6 μM did not stimulate the uncoupling activity on oxidative phosphorylation as highlighted by unresponsive oxygen consumption. The unusual effect on the stimulation of maximal respiration was not related to fibronectin- or PDL-coated plates used for cellular metabolism analysis. Conversely, irradiation of frozen–thawed bovine sperm with the red light improved mitochondrial parameters. In effect, the maximal respiration of red-light-stimulated sperm in PDL-coated plates was higher than the non-irradiated. In spite of this, red-light irradiation had no impact on membrane integrity and mitochondrial activity evaluated by epifluorescence microscopy. |
| Keywords: | bioenergetics mitochondria red-light stimulation spermatozoa |