| 利用ISSR分子标记对39份莲藕种质资源的遗传多样性分析 |
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| 引用本文： | 李长春,戴余军,苏超,李建华,田春元,姚国新. 利用ISSR分子标记对39份莲藕种质资源的遗传多样性分析[J]. 北方园艺, 2011, 0(12): 96-98 |
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| 作者姓名： | 李长春 戴余军 苏超 李建华 田春元 姚国新 |
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| 作者单位： | 孝感学院生命科学技术学院,湖北,孝感,432000;孝感学院生命科学技术学院,湖北,孝感,432000;孝感学院生命科学技术学院,湖北,孝感,432000;孝感学院生命科学技术学院,湖北,孝感,432000;孝感学院生命科学技术学院,湖北,孝感,432000;孝感学院生命科学技术学院,湖北,孝感,432000 |
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| 基金项目： | 湖北省教育厅优秀中青年人才资助项目(Q20102701); 湖北省农业资源利用重点(培育)学科资助项目(鄂学位〔2010〕1号) |
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| 摘 要： | 采用ISSR分子标记技术对39份莲藕品种进行遗传多样性分析。结果表明:8个ISSR引物共扩增出89条带,其中有55条多态带,平均每个引物扩增的多态性带数为6.88条,多态性比率平均为61.8%;通过遗传相似系数和聚类分析,能将39份莲藕品种完全区分开;说明ISSR标记技术能较好地从分子水平揭示出莲藕品种的遗传多样性。
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| 关 键 词： | 莲藕 遗传多样性 ISSR |
Analysis of Genetic Diversity of 39 Lotus Root Cultivars by Using ISSR Markers |
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| LI Chang-chun,DAI Yu-jun,SU Chao,LI Jian-hua,TIAN Chun-yuan,YAO Guo-xin. Analysis of Genetic Diversity of 39 Lotus Root Cultivars by Using ISSR Markers[J]. Northern Horticulture, 2011, 0(12): 96-98 |
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| Authors: | LI Chang-chun DAI Yu-jun SU Chao LI Jian-hua TIAN Chun-yuan YAO Guo-xin |
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| Affiliation: | LI Chang-chun,DAI Yu-jun,SU Chao,LI Jian-hua,TIAN Chun-yuan YAO Guo-xin(College of Life Science and Technology,Xiaogan University,Xiaogan,Hubei 432000) |
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| Abstract: | ISSR were used to detect the genetic diversity among 39 lotus root cultivars and 89 bands were amplified with 8 primers,including 55 polymorphic bands.The polymorphic percentage was 61.8%.And all the 39 lotus root cultivars could be distinguished by genetic similarity coefficient and cluster analysis.The result showed that ISSR approach was efficient to reveal the genetic relationship of lotus root on molecular level,which could provid reference for research in lotus root ermplasm. |
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| Keywords: | lotus root polymorphism ISSR |
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