| 北五加皮不同时间水提物中绿原酸含量的变化研究 |
| |
| 引用本文： | 关颖丽,蔡黎明,杨荣艳.北五加皮不同时间水提物中绿原酸含量的变化研究[J].北方园艺,2011(10):154-155. |
| |
| 作者姓名： | 关颖丽 蔡黎明 杨荣艳 |
| |
| 作者单位： | 1. 通化师范学院,制药与食品科学系,吉林,通化,134002
2. 吉林省四平市食品药品检验所,吉林,通化,136001 |
| |
| 基金项目： | 吉林省教育厅科研基金资助项目(吉教科合字[2009]第464号) |
| |
| 摘 要： | 利用HPLC法研究了北五加皮不同时间水提物中绿原酸含量的变化规律。采用色谱柱Agilent ZORBAX SB-C18(150mm×4.6mm,5μm),流动相为0.4%磷酸溶液(A)-乙腈(B),梯度洗脱0～8min 24%B;8～12min 50%B],检测波长278nm,流速为1.0mL/min,柱温为25℃。结果表明:绿原酸的最佳提取时间为2h,该方法简单快速、可行,含量测定准确。
|
| 关 键 词： | 北五加皮 绿原酸 HPLC |
Study on the Content Change of the Chlorogenic Acid in Water Extract of Cortex periplocae at the Different Times |
| |
| GUAN Ying-li,CAI Li-ming,YANG Rong-yan.Study on the Content Change of the Chlorogenic Acid in Water Extract of Cortex periplocae at the Different Times[J].Northern Horticulture,2011(10):154-155. |
| |
| Authors: | GUAN Ying-li CAI Li-ming YANG Rong-yan |
| |
| Institution: | GUAN Ying-li~1,CAI Li-ming~1,YANG Rong-yan~2 (1.Department of Pharmaceutics and Food Science,Tonghua Teachers College,Tonghua,Jilin 134002,2.Siping Institute of Pharmaceutical and Food,Siping,Jilin 136001) |
| |
| Abstract: | The content change of chlorogenic acid in water extract of Cortex periplocae at the different times were determined by HPLC.The ZORBAX SB-C18 columm(150 mm×4.6 mm,5μm)was used,the mobile phase was 0.4%phosphoric acid solution(A)-methyl cyanide(B),the gradient elution0～8 min 24%B;8～12 min 50%B]. The flow rate was 1.0 mL/min,the detection wavelength was 278 nm.The temperature of column was 25℃.The results showed that two hours was the best time to extraction of chlorogenic acid.The method was simple,fast,feasible and accurate. |
| |
| Keywords: | Cortex periplocae chlorogenic acid HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
