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响应面法优化超声提取柚皮总黄酮工艺的研究
引用本文:于桂香,于咏梅,张中华,俞守能,刘建萍.响应面法优化超声提取柚皮总黄酮工艺的研究[J].北方园艺,2011(8):23-26.
作者姓名:于桂香  于咏梅  张中华  俞守能  刘建萍
作者单位:中国农业大学,烟台研究院,山东,烟台,264670;烟台市土地资产经营中心,山东,烟台,264000
基金项目:中国农业大学烟台研究院科研基金资助项目(2010)
摘    要:利用超声波法提取蜜柚果皮中的总黄酮,在单因素试验的基础上,采用响应面法对主要工艺参数进行优化并得到回归模型。结果表明:回归模型较好地反映了黄酮得率与乙醇浓度、提取温度、超声时间的关系;最佳工艺条件为乙醇浓度81%、提取温度61.5℃、超声时间45min。在此工艺条件下,蜜柚果皮黄酮得率为6.667mg/g,与回归模型预测值的相对误差为0.01%。

关 键 词:柚皮  黄酮  超声提取  参数优化  响应面法

Optimization of Ultrasonic-Assisted Extraction of Total Flavones from Citrus grandis Peel by Response Surface Analysis
YU Gui-xiang,YU Yong-mei,ZHANG Zhong-hua,YU Shou-neng,LIU Jian-ping.Optimization of Ultrasonic-Assisted Extraction of Total Flavones from Citrus grandis Peel by Response Surface Analysis[J].Northern Horticulture,2011(8):23-26.
Authors:YU Gui-xiang  YU Yong-mei  ZHANG Zhong-hua  YU Shou-neng  LIU Jian-ping
Institution:YU Gui-xiang~1,YU Yong-mei~1,ZHANG Zhong-hua~2,YU Shou-neng~1,LIU Jian-ping~1 (1.Yantai Academy of China Agricultural University,Yantai,Shandong 264670,2.Yantai Municipal Land Assets Management Center,Shandong 264000)
Abstract:Using method of ultrasonic to total flavonoids from Citrus grandis were extracted based on the single factor test,by the methods of response surface to key process parameters were optimizated and acquire regression model.The results showed that better reflects the relationship of the flavonoids yield and ethanol concentration,extraction temperature,extraction time.The ethanol concentration of 81%,extraction temperature of 61.5℃,extraction time 45 min were optimum conditions.Under this condition,the pomelo peel flavonoids yield was 6.667 mg/g,relative error was 0.01%than predicted value of regression model.
Keywords:Citrus grandis peel  flavones  ultrasonication extraction  parameter optimization  method of response surface  
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