| 百子莲组织培养及植株再生研究 |
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| 引用本文： | 胡仲义,何月秋.百子莲组织培养及植株再生研究[J].北方园艺,2011(10):118-120. |
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| 作者姓名： | 胡仲义 何月秋 |
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| 作者单位： | 宁波城市职业技术学院,环境学院,浙江,奉化,315502;宁波城市职业技术学院,环境学院,浙江,奉化,315502 |
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| 摘 要： | 以百子莲花蕾为外植体,成功建立了其离体再生体系。结果表明:百子莲花蕾在诱导培养基MS+6-BA 4mg/L+NAA 0.1mg/L可分化出不定芽;在MS+6-BA 2mg/L+NAA 0.2mg/L+GA3 0.1mg/L培养基上,百子莲不定芽增殖倍数可达到5.07;百子莲不定芽适宜的生根培养基为1/2MS+NAA 0.1mg/L+AC 0.1g/L,生根率可达100%,且百子莲组培苗移栽1个月后成活率可达到95%以上。
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| 关 键 词： | 百子莲 花蕾 不定芽 植株再生 |
Study on Tissue Culture and Plantlet Regeneration of Agapanthus praecox ssp.Orientalis 'Big Blue' |
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| HU Zhong-yi,HE Yue-qiu.Study on Tissue Culture and Plantlet Regeneration of Agapanthus praecox ssp.Orientalis 'Big Blue'[J].Northern Horticulture,2011(10):118-120. |
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| Authors: | HU Zhong-yi HE Yue-qiu |
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| Institution: | HU Zhong-yi,HE Yue-qiu (College of Environmental,Ningbo City College of Vocational Technology,Fenghua,Zhejiang 315502) |
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| Abstract: | An in vitro plant regeneration system of Agapanthus praecox ssp.Orientalis 'Big Blue' was established using buds as explants.The results showed that the suitable adventitious buds inducing medium was murashige and skoog (MS)+NAA 0.1 mg/L+6-BA 4.0 mg/L.Adventitious buds were effectively generated and proliferated on MS+ 6-BA 2.0 mg/L+NAA 0.2 mg/L+GA_3 0.1 mg/L,which the multiple for multiplication was up to 5.08.The best rooting medium was 1/2MS+NAA 0.1 mg/L+AC 0.1 g/L with a rooting rate 100%.The plantlet w... |
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| Keywords: | Agapanthus praecox ssp Orientalis 'Big Blue' buds adventitious buds regeneration system |
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