| 酶法提取云南萝芙木中阿吗碱的研究 |
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| 引用本文： | 董旭杰,曹福祥,邓明,龙绛雪.酶法提取云南萝芙木中阿吗碱的研究[J].北方园艺,2011(12):48-50. |
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| 作者姓名： | 董旭杰 曹福祥 邓明 龙绛雪 |
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| 作者单位： | 中南林业科技大学生命科学与技术学院,湖南,长沙,410004 |
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| 基金项目： | 湖南省教育厅学科带头人培养对象基金资助项目(2004559); 云南萝芙木良种培育和规范种植关键技术开发项目(云发改高科20071718号) |
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| 摘 要： | 研究3种纤维素酶法提取云南萝芙木中阿吗碱的最佳工艺。结果表明:酶解最适条件为:pH 5.0,酶用量5 mg/5g,时间2 h,温度50℃;酶解处理后的阿吗碱提取率比直接用甲醇提取提高了29.3%;说明酶解处理更有利于阿吗碱的提取。
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| 关 键 词： | 阿吗碱 纤维素酶 云南萝芙木 |
Study on Extraction Ajmalicine from Rauvolfia yunnanensis Tsiang by Cellulose |
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| DONG Xu-jie,CAO Fu-xiang,Deng Ming,LONG Jiang-xue.Study on Extraction Ajmalicine from Rauvolfia yunnanensis Tsiang by Cellulose[J].Northern Horticulture,2011(12):48-50. |
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| Authors: | DONG Xu-jie CAO Fu-xiang Deng Ming LONG Jiang-xue |
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| Institution: | DONG Xu-jie,CAO Fu-xiang,Deng Ming,LONG Jiang-xue(Central South University of Forestry and Technology,Changsha,Hunan 410004) |
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| Abstract: | To study the optimal enzymatic extraction of ajmalicine from Rauvolfia yunnanensis Tsiang.The results showed that the best enzymatic hydrolysis conditions for extracting ajmalicine were as follows:pH value of enzyme solution of 5.0,the enzyme dosage of 5 mg/5g,the reaction time of 2 h,and the reaction temperature of 50℃.Compared with the MeOH extraction method,the extraction yield of ajmalicine with cellulose increased by 29.3%. |
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| Keywords: | ajmalicine cellulose Rauvolfia yunnanensis Tsiang |
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