| 油松成熟胚子叶-胚轴段的离体培养与不定芽诱导 |
| |
| 引用本文： | 马佩,唐德瑞,王永波.油松成熟胚子叶-胚轴段的离体培养与不定芽诱导[J].北方园艺,2011(7):114-117. |
| |
| 作者姓名： | 马佩 唐德瑞 王永波 |
| |
| 作者单位： | 西北农林科技大学林学院; |
| |
| 基金项目： | 国家林业局“948”资助项目(980405) |
| |
| 摘 要： | 以油松成熟胚的子叶-胚轴段为起始外植体,进行离体培养诱导不定芽,以不同浓度的活性炭对油松不定芽进行继代培养。结果表明:子叶-胚轴段可以诱导出不定芽;在1/2MS培养基上附加3 mg/L的6-BA和0.5 mg/L的NAA,外植体不定芽的诱导率达46.7%,平均增殖系数是10,最大增殖系数为24;1/2MS培养基中附加2 mg/L的活性炭对油松不定芽增殖和生长最有利。
|
| 关 键 词： | 油松 子叶-胚轴段 不定芽 活性炭 |
In Vitro Formation of Adventitious Buds From the Embryos' Cotyledon-epicotyl of Pinus tabulae formis and Adventitious Buds Culture |
| |
| MA Pei,TANG De-rui,WANG Yong-bo.In Vitro Formation of Adventitious Buds From the Embryos' Cotyledon-epicotyl of Pinus tabulae formis and Adventitious Buds Culture[J].Northern Horticulture,2011(7):114-117. |
| |
| Authors: | MA Pei TANG De-rui WANG Yong-bo |
| |
| Institution: | MA Pei,TANG De-rui,WANG Yong-bo(College of Forestry,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100) |
| |
| Abstract: | The mature embryos'cotyledon-epicotyl of Pinus tabulaeformis were used as explants to induce adventitious buds and the differents concentration of activated charcoal on Pinus tabulaeformis adventitious buds culturelation.The results showed that the cotyledon-epicotyl of mature embryos can induce adventitious buds,the optimal combination of the media was 1/2MS with 3 mg/L 6-BA and 0.5 mg/L NAA,on which 46.7% explants was induced,average rate of propagation was 10,and the highest rate of propagation was 24.Th... |
| |
| Keywords: | Pinus tabulaeformis cotyledon-epicotyl adventitious bud activated charcoal |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
