| 水毛茛组织培养研究 |
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| 引用本文： | 陈泽英,普晓兰,林萍,吴荣,陶顺江,张元伟.水毛茛组织培养研究[J].北方园艺,2011(8):161-163. |
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| 作者姓名： | 陈泽英 普晓兰 林萍 吴荣 陶顺江 张元伟 |
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| 作者单位： | 西南林业大学,园林学院,云南,昆明,650224;西南林业大学,资源学院,云南,昆明,650224 |
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| 摘 要： | 以水毛茛带腋芽茎段为外植体进行离体培养研究。结果表明:带腋芽茎段在6-BA1.0mg/L+IBA 0.1mg/L激素配比下能直接诱导出大量生长健壮的丛生芽,诱导率达66.7%。最佳生根培养基为1/2MS+IBA 2.0mg/L,生根率达72.0%。对培养方式和培养温度研究得出,液体培养比固体培养更有利于丛芽的诱导和生长,较适合的培养温度为25℃。生根苗练苗3 d后,移入自来水中,成活率达63.3%。
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| 关 键 词： | 水毛茛 丛生芽 液体培养 固体培养 |
Study on Tissue Culture of Batrachium bungei |
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| CHEN Ze-ying,PU Xiao-lan,LIN Ping,WU Rong,TAO Shun-jiang,ZHANG Yuan-wei.Study on Tissue Culture of Batrachium bungei[J].Northern Horticulture,2011(8):161-163. |
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| Authors: | CHEN Ze-ying PU Xiao-lan LIN Ping WU Rong TAO Shun-jiang ZHANG Yuan-wei |
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| Institution: | CHEN Ze-ying~1,PU Xiao-lan~2,LIN Ping~1,WU Rong~1,TAO Shun-jiang~2,ZHANG Yuan-wei~2 (1.College of Landscape Architecture,Southwest Forestry University,Kunming,Yunnan 650224,2.College of Natural Resouces,Yunnan 650224) |
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| Abstract: | Taking stem segments with axillary buds of Batrachium bungei as explants,by the method of isolated culture was studied.The cluster buds was induced by stem with auxiliary bud in the medium of MS with 1.0 mg/L 6-BA and 0.1 mg/L IBA,and the induction rate reached 66.7%.The optimal culture medium for rooting was 1/2MS with 2.0 mg/L IBA,and the induction rate reached 72.0%.Studied culture methods and culture temperature,Results showed that liquid culture was more conducive than solid culture to the induction an... |
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| Keywords: | Batrachium bungei buds liquid culture solid culture |
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