| 南岭莪术的组织培养技术研究 |
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| 引用本文： | 张施君,刘念,盛爱武,吴国江.南岭莪术的组织培养技术研究[J].北方园艺,2011(8):151-153. |
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| 作者姓名： | 张施君 刘念 盛爱武 吴国江 |
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| 作者单位： | 仲恺农业工程学院,园艺与园林学院,广东,广州,510225;华南植物园,广东,广州,510650 |
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| 基金项目： | 广东省科技攻关资助项目(2008B020400001); 中科院百人才计划资助项目 |
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| 摘 要： | 以南岭莪术根茎上的芽为外植体,采用不同激素浓度的培养基对其进行了芽诱导、丛生芽继代、试管苗生根等研究。结果表明:在MS培养基中添加TDZ 0.05mg/L时,不定芽的诱导率最高,达到90%;丛生芽的增殖培养以MS+TDZ 0.3mg/L培养基为最佳,增殖倍数达到15.8,同时在芽的基部自发形成不定根;将长约3～4cm的生根苗切出,转入1/2MS基本培养基上壮苗,约4周后即可出瓶移栽,成活率达98%以上。
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| 关 键 词： | 南岭莪术 组织培养 芽 生根 |
Study on Tissue Culture of Curcuma kwangsiensis vat.nanlingensis N.Liu et X.Y.Ma |
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| ZHANG Shi-jun,LIU Nian,SHENG Ai-wu,WU Guo-jiang.Study on Tissue Culture of Curcuma kwangsiensis vat.nanlingensis N.Liu et X.Y.Ma[J].Northern Horticulture,2011(8):151-153. |
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| Authors: | ZHANG Shi-jun LIU Nian SHENG Ai-wu WU Guo-jiang |
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| Institution: | ZHANG Shi-jun~1,LIU Nian~1,SHENG Ai-wu~1,WU Guo-jiang~2 (1.College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225,2.South China Botanical Garden,Guangdong 510650) |
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| Abstract: | An in vitro protocol for plantlet regeneration of Curcuma kwangsiensis var nanlingensis had been optimized. Adventitious shoots were induced by culture of bud explants for 2 weeks in MS medium supplemented with 0.05 mg/L TDZ.The regeneration rate was up to 15.8 shoots/explant on MS medium with 0.3 mg/L TDZ.Rooting was spontaneous and the regenerated plants were transplanted in 1/2 MS basal medium for further development.Then the in vitro induced plants were successfully transferred to sand with 98%survival ... |
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| Keywords: | Curcuma kwangsiensis var nanlingensis tissue culture axillary bud rooting |
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