| 山东省葡萄几种主要病毒病调查及检测研究 |
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| 引用本文： | 王升吉,赵玖华,尚佑芬,吕志华,张加魁,赵亚,戴争. 山东省葡萄几种主要病毒病调查及检测研究[J]. 北方园艺, 2011, 0(7): 20-23 |
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| 作者姓名： | 王升吉 赵玖华 尚佑芬 吕志华 张加魁 赵亚 戴争 |
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| 作者单位： | 山东省农业科学院,植物保护研究所,山东省植物病毒学重点实验室,山东,济南,250100;济宁市林业局经济林站,山东,济宁,272000;山东省酿酒葡萄科学研究所,山东,济南,250100 |
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| 基金项目： | 山东省自然科学基金资助项目(Y2006D32) |
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| 摘 要： | 对采自山东省不同葡萄园类别的葡萄样品,采用PTA-ELISA法和Dot-ELISA法对葡萄茎痘相关病毒(GRSPaV)进行了检测,采用DAS-ELISA法对葡萄卷叶病的3个毒原(GL-RaV-1-、2-、3)进行了检测。GRSPaV检测结果表明:检测的61个品种518个样品,阳性样品率33.6%,阳性品种率73.8%,其中资源圃样品阳性率32.7%,生产园样品阳性率34.0%。对检测到阳性样品辅以RT-PCR法验证,2对引物均扩增出了预期片段,分别为解旋酶基因340 bp片段、CP基因842 bp片段。GLRaV-1-、2-、3的检测结果表明:鲜食品种3种病原的阳性样品率分别为33.7%、7.1%、62.2%,酿酒品种阳性样品率分别为35.1%、16.2%、54.1%,综合3种病毒的阳性样品率为76.3%,品种感染率87.7%。通过该类研究对推动国内葡萄无毒化生产有重要意义。
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| 关 键 词： | 葡萄茎痘相关病毒(GRSPaV) 葡萄卷叶病毒-1、-2、-3(GLRaV-1、-2、-3) 检测 山东 |
Research on the Survey and Detection of Several Kinds of Main Grapevine Viruses in Shandong Province |
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| WANG Sheng-ji,ZHAO Jiu-hua,SHANG You-fen,LV Zhi-hua,ZHANG Jia-kui,ZHAO Ya,DAI Zheng. Research on the Survey and Detection of Several Kinds of Main Grapevine Viruses in Shandong Province[J]. Northern Horticulture, 2011, 0(7): 20-23 |
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| Authors: | WANG Sheng-ji ZHAO Jiu-hua SHANG You-fen LV Zhi-hua ZHANG Jia-kui ZHAO Ya DAI Zheng |
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| Affiliation: | WANG Sheng-ji1,ZHAO Jiu-hua1,SHANG You-fen1,LV Zhi-hua2,ZHANG Jia-kui3,ZHAO Ya1,DAI Zheng1(1.Institute of Plant Protection,Academy of Agricultural Science of Shandong,Plant Virology Critical Laboratory of Shandong,Jinan,Shandong 250100,2.Economic Forest Station of Forest Bureau of Jining,Jining,Shandong 272000,3.Institute of Wine Grape Science of Shandong,Shandong 250100) |
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| Abstract: | The study had detected grapevine rupestris stem pitting associated virus(GRSPaV) of grapevine samples collected from different vineyards category in Shandong province,with PAT-ELISA and Dot-ELISA.Meanwhile,3 types of toxogenes of grapevine leafroll associated virus-1,-2,-3(GLRaV-1,-2,-3) had also been detected with DAS-ELISA.The results of GRSPaV detection were,among 518 samples of 61 cultivars,33.6% sample positive rate,73.8% cultivar positive rate;the sample positive rate of grapevine germplasm,production... |
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| Keywords: | grapevine rupestris stem pitting associated virus(GRSPaV) grapevine leafroll associated virus-1,■2,■3(GLRaV-1,■,■3) detection Shandong province |
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