| '霍巴'海棠组培快繁技术研究 |
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| 引用本文： | 裴轶琨,李晓玲,李克秀,李毅丹. '霍巴'海棠组培快繁技术研究[J]. 北方园艺, 2011, 0(10): 124-127 |
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| 作者姓名： | 裴轶琨 李晓玲 李克秀 李毅丹 |
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| 作者单位： | 长春工业大学,化学与生命科学学院,吉林,长春,130012;吉林省农业科学院,吉林,长春,130033 |
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| 摘 要： | 以‘霍巴’海棠(Malus‘Hopa’)的茎尖和带腋芽的幼嫩茎段为外植体,建立了比较高效的‘霍巴’海棠组培快繁体系。结果表明:‘霍巴’海棠茎尖及茎段外植体在MS+6-BA0.1mg/L+IAA 0.2mg/L+3%蔗糖+0.48%琼脂(pH 5.8)培养基上生长状况较好;将其组培苗在WPM+6-BA 0.5mg/L+NAA 0.1mg/L+ZT 0.01mg/L+3%蔗糖+0.48%琼脂(pH 5.8)培养基上继代培养40d左右,继代增殖系数均可达到3.0左右;再经1/2 WPM+NAA0.8mg/L+1.5%蔗糖+0.48%琼脂(pH 5.8)培养基生根壮苗后,移栽成活率可达97.5%。
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| 关 键 词： | 霍巴海棠 组织培养 快速繁殖 |
Study on Rapid Propagation of Malus 'Hopa' by Tissue Culture |
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| PEI Yi-kun,LI Xiao-ling,LI Ke-xiu,LI Yi-dan. Study on Rapid Propagation of Malus 'Hopa' by Tissue Culture[J]. Northern Horticulture, 2011, 0(10): 124-127 |
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| Authors: | PEI Yi-kun LI Xiao-ling LI Ke-xiu LI Yi-dan |
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| Affiliation: | PEI Yi-kun~1,LI Xiao-ling~1,LI Ke-xiu~1,LI Yi-dan~2 (1.College of Chemistry and Life Science,Changchun University of Technology,Changchun,Jilin 130012,2.Biotechnology Research Centre,Jinlin Academy of Agricultural Sciences,Jilin 130033) |
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| Abstract: | An efficient protocol for the rapid propagation of Malus 'Hopa'by tissue culture was developed,with the stem apex or sections as the explants.The results showed that the above explants could grow on the primary medium contained MS basal salt s and vitamins,6-BA 0.1 mg/L,IAA 0.2 mg/L and 3%sucrose and solidified w ith 0.48% agar(pH 5.8).After the shoots in vitro were subcultured for about 40 days on the subculture medium contained WPM basal salt s and vitamins,6-BA 0.5 mg/L,NAA 0.1 mg/L,ZT 0.01 and 3%sucrose... |
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| Keywords: | Malus 'Hopa' tissue culture rapid propagation |
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