| 鸭梨Hc ISSR-PCR反应体系的优化 |
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| 引用本文： | 支婷,刘春琴,崔建州,杜克久.鸭梨Hc ISSR-PCR反应体系的优化[J].北方园艺,2011(12):89-92. |
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| 作者姓名： | 支婷 刘春琴 崔建州 杜克久 |
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| 作者单位： | 河北农业大学林学院,河北,保定,071000;沧州市农林科学院,河北,沧州,061001 |
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| 基金项目： | “十一五”国家科技支撑计划资助项目(2006BAD08A03-5); 国家公益性行业科研专项资助项目(200803011-9); 河北省林业局资助项目(0207216) |
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| 摘 要： | 应用正交设计的方法对影响鸭梨Hc ISSR-PCR反应的4个因素(模板DNA、引物、dNTPs、TaqDNA聚合酶)进行5个水平的优化试验,以DPS 7.55软件分析。结果表明:鸭梨HcISSR-PCR的最佳反应体系(20μL)为:模板DNA 60 ng、引物浓度0.4μmol/L、dNTPs浓度0.1mmol/L、TaqDNA聚合酶2 U。
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| 关 键 词： | 鸭梨Hc ISSR-PCR反应体系 正交设计 优化 |
Optimization of ISSR-PCR Reaction System of Pyrus bretschneideri Rehd.cv.Yali Hc |
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| ZHI Ting,LIU Chun-qin,CUI Jian-zhou,DU Ke-jiu.Optimization of ISSR-PCR Reaction System of Pyrus bretschneideri Rehd.cv.Yali Hc[J].Northern Horticulture,2011(12):89-92. |
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| Authors: | ZHI Ting LIU Chun-qin CUI Jian-zhou DU Ke-jiu |
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| Institution: | ZHI Ting1,LIU Chun-qin2,CUI Jian-zhou1,DU Ke-jiu1(1.College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071000,2.Cangzhou Academy of Agriculture and Forestry Science,Cangzhou,Hebei 061001) |
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| Abstract: | The orthogonal design was used to optimize ISSR-PCR amplification system of Pyrus bretschneideri Rehd.cv.Yali Hc in four factors(DNA template,primer,dNTPs,and Taq DNA polymerase)at five levels respectively.The results of PCR was analyzed by software DPS 7.55,and the best level of each factor were selected.The most suitable ISSR-PCR reaction system was 60 ng DNA template,0.4μmol/L primer,0.1mmol/L dNTPs,2.0U Taq DNA polymerase in 20 μL reaction system. |
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| Keywords: | Pyrus bretschneideri Rehd cv Yali Hc ISSP-PCR reaction system orthogonal design optimization |
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