| 西伯利亚远志组织培养与植株再生研究 |
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| 引用本文： | 任建伟,魏常燕,王进茂. 西伯利亚远志组织培养与植株再生研究[J]. 北方园艺, 2011, 0(12): 106-109 |
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| 作者姓名： | 任建伟 魏常燕 王进茂 |
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| 作者单位： | 河北农业大学林学院,河北,保定,071000;河北农业大学林学院,河北,保定,071000;河北农业大学林学院,河北,保定,071000 |
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| 摘 要： | 以西伯利亚远志带腋芽的茎段为外植体,探讨不同激素处理6-BAI、BA、NAA组合对西伯利亚远志试管苗增殖及生根的影响,建立一套西伯利亚远志离体培养技术体系。结果表明:适合西伯利亚远志增殖培养基为MS+6-BA 0.5 mg/L+IBA 0.1 mg/L,生根培养基为1/2MS+IBA 1.0 mg/L,在此体系中增殖系数达3.15,生根率100%,移栽成活率可达100%。
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| 关 键 词： | 西伯利亚远志 组织培养 快速繁殖 |
Research on the Tissue Culture and Plant Regenetation of Polygala sibirica L. |
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| REN Jian-wei,WEI Chang-yan,WANG Jin-mao. Research on the Tissue Culture and Plant Regenetation of Polygala sibirica L.[J]. Northern Horticulture, 2011, 0(12): 106-109 |
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| Authors: | REN Jian-wei WEI Chang-yan WANG Jin-mao |
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| Affiliation: | REN Jian-wei,WEI Chang-yan,WANG Jin-mao(College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071000) |
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| Abstract: | Stems with axillaries bud of Polygala sibirica L.were used as explants,and placed on MS culture medium with different hormone of 6-BA,IBA,NAA combination and different concentrations,and established a new technical system of plantlet regeneration and rapid propagation.The results showed that the optimum multiplication culture medium was MS+6-BA 0.5 mg/L+IBA 0.1 mg/L;the rooting culture medium was 1/2MS+IBA 1.0 mg/L.In this system,the highest propagation coefficient was 3.15,the rooting rate was 100%,and the... |
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| Keywords: | Polygala sibirica L. tissue culture rapid propagation |
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