单细胞凝胶电泳检测百草枯对大鳞副泥鳅血细胞DNA的损伤

以大鳞副泥鳅（Paramisgurnus dabryanus）为受试对象,设置0.302、0.362、0.434、0.521、0.625 mL/L共5个浓度组和1个对照组,通过单细胞凝胶电泳技术（SCGE）,研究除草剂百草枯对大鳞副泥鳅血细胞DNA 6、24、48、96 h所造成的损伤。结果表明,6、24、48、96 h时处理组与对照组相比,在尾部DNA百分比（Tail DNA）、慧星尾长（Tail length,TL）和Olive尾矩（Olive tail moment,OTM）3项指标上均具有显著差异（P〈0.05）,而且在6、24、48 h时,时间一定时,各项指标随除草剂浓度升高而变大;浓度一定时,各项指标随时间推移而变大,而96 h时与48 h时相比,各项指标则有所下降。TL和OTM 2项指标在24 h时0.625 mL/L组达到最大,分别为（553.22±106.92）%和（200.73±50.72）%,Tail DNA在48 h时0.625 mL/L组达到最大,为（75.14±10.99）%。研究结果对于百草枯的合理使用和环境污染监测提供了科学依据,也为其对水生生物的毒性研究提供了资料。

The DNA Damage Effect of Paraquat on Blood Red Cell of Paramisgurnus dabryanus

We studied the DNA damage effect of paraquat at different time （6,24,48,96 h） and different doses （0. 302,0. 362,0. 434,0. 521,0. 625 mL/L and CK） on Paramisgurnus dabryanus. SCGE was applied to test DNA damage. For each comet, Tail DNA, Tail Length, Olive Tail Moment was measured with CASP and the data was analyzed with SPSS 13.0 for windows. The results showed that there was significant difference between groups with 6, 24,48,96 h treatment of paraquat and CK（P 〈0.05） in Tail DNA, Tail Length, Olive Tail Moment. Moreover, at identical time, a dose-dependent increase in Tail DNA, Tail Length, Olive Tail Moment in groups of 6,24,48 h treatment was observed and a time-dependent inerease in Tail DNA,Tail Length, Olive Tail Moment was observed at identical dose in groups of 0. 302,0. 362,0. 434,0. 521 mL/L treatment in general. However, there was a decrease＇ in Tail DNA,Tail Length,Olive Tail Moment at 96 h eompared to 48 h group, at 24 h treatment of 0 625ml/L dose Tail Length,Olive Tail Moment reached their maximum levels at （553.22 ±106.92） % and （200.73 ±50.72）% ; at 48 h treatment of 0. 625 mL/L dose Tail DNA reached its maximum level at （75.14 ± 10.09）%.