| 扁桃SFB基因的克隆及其生物信息学分析 |
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| 引用本文： | 郭长奎,罗淑萍,何天明,李疆,高启明.扁桃SFB基因的克隆及其生物信息学分析[J].果树学报,2009,26(2). |
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| 作者姓名： | 郭长奎 罗淑萍 何天明 李疆 高启明 |
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| 作者单位： | 郭长奎,罗淑萍,GUO Chang-kui,LUO Shu-ping(新疆农业大学农学院);何天明,李疆,高启明,HE Tian-ming,LI Jiang,GAO Qi-ming(新疆农业大学林学与园艺学院,新疆农业大学特色果树研究中心,乌鲁木齐,830052)
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| 基金项目： | 国家自然科学基金，国家科技支撑项目 |
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| 摘 要： | 以新疆扁桃栽培品种麻壳的花药为材料,利用RT-PCR和RACE技术,克隆到了一个全长为1131bp的SFB基因(Genbank登录号为:EU909685),命名为AcSFB10。该基因编码376个氨基酸,在N末端含有一个大约50个氨基酸组成的F-box基序。经生物信息学分析,推测AcSFB10蛋白的分子式为C2000H3033N517O558S23,相对分子质量为43985.5,等电点为6.02,二级结构以α螺旋为主;理论推导半衰期大约为30h,不稳定参数为53.21,属于不稳定蛋白;并且推测此蛋白为亲水性、非分泌型蛋白,在基质内起裂合酶的作用,特异性的识别底物,正好吻合了F-box蛋白的作用。
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| 关 键 词： | 扁桃 自交不亲和 SFB基因 生物信息学 |
Cloning and bioinformatic analysis of SFB gene in almond |
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| GUO Chang-kui,LUO Shu-ping,HE Tian-ming,LI Jiang,GAO Qi-ming.Cloning and bioinformatic analysis of SFB gene in almond[J].Journal of Fruit Science,2009,26(2). |
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| Authors: | GUO Chang-kui LUO Shu-ping HE Tian-ming LI Jiang GAO Qi-ming |
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| Institution: | 1College of Agronomy;Xinjiang Agricultural University;Urumqi;Xinjiang 830052 China;2College of Forestry and Horticulture;Research Centre of characteristic Fruit;Xinjiang 830052 China |
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| Abstract: | A total length SFB gene(Genbank accession number:EU909685) named AcSFB10 was cloned with RT-PCR and RACE technology from the anther of almond planted in Xinjiang.AcSFB10 was 1131 bp in length encoding a protein of 376 amino acids,which had an F-box motif containing about 50 amino acids in the N-terminatio.With the bioinformatics analy-sis,the predicted AcSFB10 protein's molecular formula was C2000H3033N517O558S23;its relative molecular mass was 43985.5,isoelectric point was 6.02 and its secondary structure ... |
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| Keywords: | Almond Self-incompatibility SFB gene Bioinformatics |
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