| 猪生殖与呼吸综合征病毒JK-100弱毒株生物学和血清学特性研究 |
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| 引用本文： | 杜文金,张德鸿,王志亮,孙融冰,宋翠平,刘卫,况慧星. 猪生殖与呼吸综合征病毒JK-100弱毒株生物学和血清学特性研究[J]. 中国预防兽医学报, 2000, 0(Z1) |
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| 作者姓名： | 杜文金 张德鸿 王志亮 孙融冰 宋翠平 刘卫 况慧星 |
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| 摘 要： | 通过ＩＦＡ、ＶＮＴ和Ｗｅｓｔｅｒｎ－Ｂｌｏｔ等血清学试验及电镜观察，证明ＪＫ－１００株为ＰＲＲＳＶ。本病毒感染ＭＡＲＣ１４５细胞后，３６小时的ＴＣＩＤ_(50)最高，为１０~(-7.83)。把本病毒分别保存在４℃、３７℃各９天，保存在４℃９天后，ＴＣＩＤ_(50)从１０~(-7.83)下降至１０~(-7.32)，３７℃７天已测不到效价。
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| 关 键 词： | 猪生殖-呼吸综合征病毒 JK-100弱毒株 |
Biological and Serological Characters of Modified Porcine Reproductive and Respiratory Syndrome Virus JK-100 Strain |
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| DU Wenjin,ZHANG Dehoeng,WANG Zhiliang,SUN Rongbing,SONG Cuiping,LIU Wei,KWANG Hueixing. Biological and Serological Characters of Modified Porcine Reproductive and Respiratory Syndrome Virus JK-100 Strain[J]. Chinese Journal of Preventive Veterinary Medicine, 2000, 0(Z1) |
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| Authors: | DU Wenjin ZHANG Dehoeng WANG Zhiliang SUN Rongbing SONG Cuiping LIU Wei KWANG Hueixing |
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| Abstract: | The JK-100 strain was identified as PRRSV by IFA,VNT,Westem-Blot and EM.The TCID50 was highest when MARC145 cell was infected by JK-100 after 36 hours.The virus was conserved under for days, it's titration reduced from 10-7.83 to 10-7.32, and under 37 for 7 days, it's titration can not be detected. |
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| Keywords: | Porcine Reproductive and Respiratory Syndrome Virus Modified JK-100 Straind |
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